biostudies-literatureUnknown7(23)Krimpenfort RAE-cadherin is a crucial regulator of epithelial cell-to-cell adhesion and an established tumor suppressor. Aside epithelia, E-cadherin expression marks the erythroid cell lineage during human but not mouse hematopoiesis. However, the role of E-cadherin in human erythropoiesis remains unknown. Because rat erythropoiesis was postulated to reflect human erythropoiesis more closely than mouse erythropoiesis, we investigated E-cadherin expression in rat erythroid progenitors. E-cadherin expression is conserved within the erythroid lineage between rat and human. In response to anemia, erythroblasts in rat bone marrow (BM) upregulate E-cadherin as well as its binding partner β-catenin. CRISPR/Cas9-mediated knock out of E-cadherin revealed that E-cadherin expression is required to stabilize β-cateBlood advances7169-7183https://www.ebi.ac.uk/biostudies/studies/S-EPMC10698263biostudies-literatureE-cadherin/β-catenin expression is conserved in human and rat erythropoiesis and marks stress erythropoiesis.PMC10698263von Lindern MKrimpenfort RAvan der Meulen SANethe MVerhagen HFilonova Gvan den Akker EHoogenboezem MDriessen MfalseE-cadherin/β-catenin expression is conserved in human and rat erythropoiesis and marks stress erythropoiesis.E-cadherin is a crucial regulator of epithelial cell-to-cell adhesion and an established tumor suppressor. Aside epithelia, E-cadherin expression marks the erythroid cell lineage during human but not mouse hematopoiesis. However, the role of E-cadherin in human erythropoiesis remains unknown. Because rat erythropoiesis was postulated to reflect human erythropoiesis more closely than mouse erythropoiesis, we investigated E-cadherin expression in rat erythroid progenitors. E-cadherin expression is conserved within the erythroid lineage between rat and human. In response to anemia, erythroblasts in rat bone marrow (BM) upregulate E-cadherin as well as its binding partner β-catenin. CRISPR/Cas9-mediated knock out of E-cadherin revealed that E-cadherin expression is required to stabilize β-cate2023-01-01T00:00:00Z2023 Dec2025-04-25T17:19:05.837Z2025-04-06T05:22:58.064ZS-EPMC106982633779279410.1182/bloodadvances.2023010875