{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Leduc A"],"funding":["NCI NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["Single-cell proteomics by mass spectrometry (MS) allows quantifying proteins with high specificity and sensitivity. To increase its throughput, we developed nPOP, a method for parallel preparation of thousands of single cells in nanoliter volume droplets deposited on glass slides. Here, we describe its protocol with emphasis on its flexibility to prepare samples for different multiplexed MS methods. An implementation with plexDIA demonstrates accurate quantification of about 3,000 - 3,700 proteins per human cell. The protocol is implemented on the CellenONE instrument and uses readily available consumables, which should facilitate broad adoption. nPOP can be applied to all samples that can be processed to a single-cell suspension. It takes 1 or 2 days to prepare over 3,000 single cells. We"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2023.11.27.568927"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10705290"],"repository":["biostudies-literature"],"pubmed_title":["Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP."],"pmcid":["PMC10705290"],"funding_grant_id":["R35 GM148218","R01 GM144967","UH3 CA268117"],"pubmed_authors":["Leduc A","Cantlon J","Koury L","Slavov N"],"additional_accession":[]},"is_claimable":false,"name":"Massively parallel sample preparation for multiplexed single-cell proteomics using nPOP.","description":"Single-cell proteomics by mass spectrometry (MS) allows quantifying proteins with high specificity and sensitivity. To increase its throughput, we developed nPOP, a method for parallel preparation of thousands of single cells in nanoliter volume droplets deposited on glass slides. Here, we describe its protocol with emphasis on its flexibility to prepare samples for different multiplexed MS methods. An implementation with plexDIA demonstrates accurate quantification of about 3,000 - 3,700 proteins per human cell. The protocol is implemented on the CellenONE instrument and uses readily available consumables, which should facilitate broad adoption. nPOP can be applied to all samples that can be processed to a single-cell suspension. It takes 1 or 2 days to prepare over 3,000 single cells. We","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2025-04-26T20:39:11.416Z","creation":"2025-04-06T16:29:30.047Z"},"accession":"S-EPMC10705290","cross_references":{"pubmed":["38076795"],"doi":["10.1101/2023.11.27.568927"]}}