biostudies-literatureUnknown11(6)Douwenga S<h4>Importance</h4>The availability of nutrients to microorganisms varies considerably between different environments, and changes can occur rapidly. As a general rule, a fast growth rate-typically growth on glucose-is associated with the repression of other carbohydrate utilization genes, but it is not clear to what extent catabolite repression is exerted by other sugars. We investigated the hierarchy of sugar utilization after substrate transitions in <i>Lactococcus cremoris</i>. For this, we determined the proteome and carbohydrate utilization capacity after growth on different sugars. The results show that the preparedness of cells for the utilization of "slower" sugars is not strictly determined by the growth rate. The data point to individual proteins relevant for various sugar transMicrobiology spectrume0224823https://www.ebi.ac.uk/biostudies/studies/S-EPMC10715065biostudies-literatureThe hierarchy of sugar catabolization in <i>Lactococcus cremoris</i>.PMC10715065Lai XVervoort JKleerebezem MBachmann HTeusink BDouwenga SBoeren Svan Olst BLuo YfalseThe hierarchy of sugar catabolization in <i>Lactococcus cremoris</i>.<h4>Importance</h4>The availability of nutrients to microorganisms varies considerably between different environments, and changes can occur rapidly. As a general rule, a fast growth rate-typically growth on glucose-is associated with the repression of other carbohydrate utilization genes, but it is not clear to what extent catabolite repression is exerted by other sugars. We investigated the hierarchy of sugar utilization after substrate transitions in <i>Lactococcus cremoris</i>. For this, we determined the proteome and carbohydrate utilization capacity after growth on different sugars. The results show that the preparedness of cells for the utilization of "slower" sugars is not strictly determined by the growth rate. The data point to individual proteins relevant for various sugar trans2023-01-01T00:00:00Z2023 Dec2026-05-29T09:17:46.34Z2025-04-04T14:31:52.842ZS-EPMC107150653788898610.1128/spectrum.02248-23