biostudies-literatureCurcio CAssociazione Italiana per la Ricerca sul CancroRotaract Club PiemonteUniversità degli Studi di TorinoFondazione Nadia ValsecchiBando CRT Città della Salute e della Scienza di TorinoGrant for InternalizationEuropean Union - Next Generation EU - NRRP M6C2 - Investment 2.1 Enhancement and strengthening of biomedical research in the NHSBando CRT Città della Salute e della Scienza di TorinoFondazione Ricerca Molinette OnlusFondazione Nastro Viola—Associazione lotta tumore al pancreas5089-5100https://www.ebi.ac.uk/biostudies/studies/S-EPMC10725354biostudies-literatureUnknown23(8)Pancreatic ductal adenocarcinoma (PDA) has a dismal prognosis due to a lack of early diagnostic markers and effective therapy. In PDA patients, the glycolytic enzyme and plasminogen receptor alpha-enolase (ENO1) and the transcription factor far upstream element-binding protein 1 (FUBP1) are upregulated and elicit the production of autoantibodies (aAb) that discriminate healthy subjects from PDA patients, with the latter mostly directed to post-translational phosphorylated isoforms. Here, the correlation of prognosis with circulating ENO1 and FUBP1aAb, and their protein tissue expression was analyzed in PDA patients. Circulating ENO1 and FUBP1 aAb was analyzed in two cohorts of PDA patients by ELISA (n = 470), while tissues expression was observed by immunohistochemistry (n = 45). Overall sClinical and experimental medicineCirculating autoantibodies to alpha-enolase (ENO1) and far upstream element-binding protein 1 (FUBP1) are negative prognostic factors for pancreatic cancer patient survival.PMC10725354CD38 and Associazione Ursula e Giorgio CytronIG no. 27020CURC_GFI_22_01_Fno. 2019.1887PNRR-POC-2022-12375658Giordano DLangella SCastellino BGuadagnin GFenocchio EFerrero ANovelli FCassoni PCiccone GArmentano SBertero LSpadi RSatolli MAMoro FBrugiapaglia SNuzzo ARosso TBellotti GDe Angelis CCurcio CPapotti MGCampra DfalseCirculating autoantibodies to alpha-enolase (ENO1) and far upstream element-binding protein 1 (FUBP1) are negative prognostic factors for pancreatic cancer patient survival.Pancreatic ductal adenocarcinoma (PDA) has a dismal prognosis due to a lack of early diagnostic markers and effective therapy. In PDA patients, the glycolytic enzyme and plasminogen receptor alpha-enolase (ENO1) and the transcription factor far upstream element-binding protein 1 (FUBP1) are upregulated and elicit the production of autoantibodies (aAb) that discriminate healthy subjects from PDA patients, with the latter mostly directed to post-translational phosphorylated isoforms. Here, the correlation of prognosis with circulating ENO1 and FUBP1aAb, and their protein tissue expression was analyzed in PDA patients. Circulating ENO1 and FUBP1 aAb was analyzed in two cohorts of PDA patients by ELISA (n = 470), while tissues expression was observed by immunohistochemistry (n = 45). Overall s2023-01-01T00:00:00Z2023 Dec2026-06-03T12:00:17.238Z2025-04-07T11:08:18.024ZS-EPMC107253543791025610.1007/s10238-023-01236-5