{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhou Y"],"funding":["the Natural Science Foundation for Universities of Jiangsu Province","the Training Program of Innovation and Entrepreneurship for College Students in Jiangsu Province"],"pagination":["233"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10728403"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["<h4>Background</h4>Celastrol has been revealed to exhibit anticancer pharmacological activity, however, the molecular mechanisms of celastrol involved in pancreatic cancer remain to be further elucidated. The present study was to illustrate whether celastrol suppresses pancreatic cancer through modulating RNA m<sup>6</sup>A modification.<h4>Methods</h4>Effect of celastrol treatment on the malignant phenotypes of pancreatic cancer cells was evaluated by CCK-8 assay, EdU assay, colony formation assay, flow cytometry analysis and subcutaneous xenograft experiments. RNA sequencing (RNA-seq) analysis was carried out to analyze the genes differentially expressed in celastrol-treated pancreatic cancer cells. RT-qPCR, Western blotting and immunohistochemistry were employed to evaluate the expressi"],"journal":["Discover oncology"],"pubmed_title":["Celastrol suppresses human pancreatic cancer via m<sup>6</sup>A-YTHDF3-mediated downregulation of Claspin and Bcl-2."],"pmcid":["PMC10728403"],"funding_grant_id":["no. 202111117038Z and 202211117033Z","20KJB310002"],"pubmed_authors":["Li H","Miao T","Chen J","Li P","Wei X","Zhou Y","Qiu Y","Tian Z","Liu Y","Zhuang H","Xu X","Wu W","Shen W","Yin J"],"additional_accession":[]},"is_claimable":false,"name":"Celastrol suppresses human pancreatic cancer via m<sup>6</sup>A-YTHDF3-mediated downregulation of Claspin and Bcl-2.","description":"<h4>Background</h4>Celastrol has been revealed to exhibit anticancer pharmacological activity, however, the molecular mechanisms of celastrol involved in pancreatic cancer remain to be further elucidated. The present study was to illustrate whether celastrol suppresses pancreatic cancer through modulating RNA m<sup>6</sup>A modification.<h4>Methods</h4>Effect of celastrol treatment on the malignant phenotypes of pancreatic cancer cells was evaluated by CCK-8 assay, EdU assay, colony formation assay, flow cytometry analysis and subcutaneous xenograft experiments. RNA sequencing (RNA-seq) analysis was carried out to analyze the genes differentially expressed in celastrol-treated pancreatic cancer cells. RT-qPCR, Western blotting and immunohistochemistry were employed to evaluate the expressi","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Dec","modification":"2026-06-11T03:18:18.97Z","creation":"2025-02-19T01:44:43.785Z"},"accession":"S-EPMC10728403","cross_references":{"pubmed":["38110764"],"doi":["10.1007/s12672-023-00838-5"]}}