biostudies-literatureUnknown12(11)Molika P<h4>Background</h4>Cervical cancer (CC) is the fourth most common cancer in females worldwide. Existing biomarkers for CC, such as squamous cell carcinoma antigens, show low specificity. Hence, a novel biomarker for the diagnosis of CC is required. Through proteomic analysis, this study aimed to distinguish between the small extracellular vesicle (sEV) protein profiles of healthy controls (HC) and CC sera and to identify potential sEV proteins that can serve as biomarkers for CC diagnosis.<h4>Methods</h4>The number and size distribution of sEVs in HC and CC sera were measured using nanoparticle tracking analysis. Differential ultracentrifugation combined with size-exclusion chromatography was used to isolate and purify sEVs. Liquid chromatography-tandem mass spectrometry was used to identiTranslational cancer research3113-3128https://www.ebi.ac.uk/biostudies/studies/S-EPMC10731333biostudies-literatureProteomic analysis of small extracellular vesicles unique to cervical cancer.PMC10731333Hanprasertpong JNavakanitworakul RMolika PLeetanaporn KRungkamoltip PRoytrakul SfalseProteomic analysis of small extracellular vesicles unique to cervical cancer.<h4>Background</h4>Cervical cancer (CC) is the fourth most common cancer in females worldwide. Existing biomarkers for CC, such as squamous cell carcinoma antigens, show low specificity. Hence, a novel biomarker for the diagnosis of CC is required. Through proteomic analysis, this study aimed to distinguish between the small extracellular vesicle (sEV) protein profiles of healthy controls (HC) and CC sera and to identify potential sEV proteins that can serve as biomarkers for CC diagnosis.<h4>Methods</h4>The number and size distribution of sEVs in HC and CC sera were measured using nanoparticle tracking analysis. Differential ultracentrifugation combined with size-exclusion chromatography was used to isolate and purify sEVs. Liquid chromatography-tandem mass spectrometry was used to identi2023-01-01T00:00:00Z2023 Nov2025-04-04T12:18:46.713Z2025-04-04T12:18:46.713ZS-EPMC107313333813031510.21037/tcr-23-517