{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang X"],"funding":["Hongliang Zhang"],"pagination":["303"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10731697"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(1)"],"pubmed_abstract":["<h4>Background</h4>Pseudorabies virus (PRV) causes substantial losses in the swine industry worldwide. Attenuated PRV strains with deletions of immunomodulatory genes glycoprotein E (gE), glycoprotein I (gI) and thymidine kinase (TK) are candidate vaccines. However, the effects of gE/gI/TK deletions on PRV-host interactions are not well understood.<h4>Methods</h4>To characterize the impact of gE/gI/TK deletions on host cells, we analyzed and compared the transcriptomes of PK15 cells infected with wild-type PRV (SD2017), PRV with gE/gI/TK deletions (SD2017gE/gI/TK) using RNA-sequencing.<h4>Results</h4>The attenuated SD2017gE/gI/TK strain showed increased expression of inflammatory cytokines and pathways related to immunity compared to wild-type PRV. Cell cycle regulation and metabolic pathw"],"journal":["Virology journal"],"pubmed_title":["Transcriptomic analysis reveals impact of gE/gI/TK deletions on host response to PRV infection."],"pmcid":["PMC10731697"],"funding_grant_id":["This research was funded by the Pig Industry Technology System of Shandong Province (Number: SDAIT-08-07) and the Shandong Key Research and Development Program, China Number: 2019GNC106074 and 2017GRC5201)."],"pubmed_authors":["Li Y","Wang C","Zhang H","Wang X","Wang Y","Dong S"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic analysis reveals impact of gE/gI/TK deletions on host response to PRV infection.","description":"<h4>Background</h4>Pseudorabies virus (PRV) causes substantial losses in the swine industry worldwide. Attenuated PRV strains with deletions of immunomodulatory genes glycoprotein E (gE), glycoprotein I (gI) and thymidine kinase (TK) are candidate vaccines. However, the effects of gE/gI/TK deletions on PRV-host interactions are not well understood.<h4>Methods</h4>To characterize the impact of gE/gI/TK deletions on host cells, we analyzed and compared the transcriptomes of PK15 cells infected with wild-type PRV (SD2017), PRV with gE/gI/TK deletions (SD2017gE/gI/TK) using RNA-sequencing.<h4>Results</h4>The attenuated SD2017gE/gI/TK strain showed increased expression of inflammatory cytokines and pathways related to immunity compared to wild-type PRV. Cell cycle regulation and metabolic pathw","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Dec","modification":"2026-05-29T10:20:46.896Z","creation":"2025-04-06T19:17:15.156Z"},"accession":"S-EPMC10731697","cross_references":{"pubmed":["38115115"],"doi":["10.1186/s12985-023-02265-y"]}}