biostudies-literatureWang XHongliang Zhang303https://www.ebi.ac.uk/biostudies/studies/S-EPMC10731697biostudies-literatureUnknown20(1)<h4>Background</h4>Pseudorabies virus (PRV) causes substantial losses in the swine industry worldwide. Attenuated PRV strains with deletions of immunomodulatory genes glycoprotein E (gE), glycoprotein I (gI) and thymidine kinase (TK) are candidate vaccines. However, the effects of gE/gI/TK deletions on PRV-host interactions are not well understood.<h4>Methods</h4>To characterize the impact of gE/gI/TK deletions on host cells, we analyzed and compared the transcriptomes of PK15 cells infected with wild-type PRV (SD2017), PRV with gE/gI/TK deletions (SD2017gE/gI/TK) using RNA-sequencing.<h4>Results</h4>The attenuated SD2017gE/gI/TK strain showed increased expression of inflammatory cytokines and pathways related to immunity compared to wild-type PRV. Cell cycle regulation and metabolic pathwVirology journalTranscriptomic analysis reveals impact of gE/gI/TK deletions on host response to PRV infection.PMC10731697This research was funded by the Pig Industry Technology System of Shandong Province (Number: SDAIT-08-07) and the Shandong Key Research and Development Program, China Number: 2019GNC106074 and 2017GRC5201).Li YWang CZhang HWang XWang YDong SfalseTranscriptomic analysis reveals impact of gE/gI/TK deletions on host response to PRV infection.<h4>Background</h4>Pseudorabies virus (PRV) causes substantial losses in the swine industry worldwide. Attenuated PRV strains with deletions of immunomodulatory genes glycoprotein E (gE), glycoprotein I (gI) and thymidine kinase (TK) are candidate vaccines. However, the effects of gE/gI/TK deletions on PRV-host interactions are not well understood.<h4>Methods</h4>To characterize the impact of gE/gI/TK deletions on host cells, we analyzed and compared the transcriptomes of PK15 cells infected with wild-type PRV (SD2017), PRV with gE/gI/TK deletions (SD2017gE/gI/TK) using RNA-sequencing.<h4>Results</h4>The attenuated SD2017gE/gI/TK strain showed increased expression of inflammatory cytokines and pathways related to immunity compared to wild-type PRV. Cell cycle regulation and metabolic pathw2023-01-01T00:00:00Z2023 Dec2026-05-29T10:20:46.896Z2025-04-06T19:17:15.156ZS-EPMC107316973811511510.1186/s12985-023-02265-y