{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cai F"],"funding":["NIDDK NIH HHS","Howard Hughes Medical Institute","NCI NIH HHS","NIGMS NIH HHS","NIH HHS"],"pagination":["1830-1843.e5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10732579"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["35(10)"],"pubmed_abstract":["Stable isotopes are powerful tools to assess metabolism. <sup>13</sup>C labeling is detected using nuclear magnetic resonance (NMR) spectroscopy or mass spectrometry (MS). MS has excellent sensitivity but generally cannot discriminate among different <sup>13</sup>C positions (isotopomers), whereas NMR is less sensitive but reports some isotopomers. Here, we develop an MS method that reports all 16 aspartate and 32 glutamate isotopomers while requiring less than 1% of the sample used for NMR. This method discriminates between pathways that result in the same number of <sup>13</sup>C labels in aspartate and glutamate, providing enhanced specificity over conventional MS. We demonstrate regional metabolic heterogeneity within human tumors, document the impact of fumarate hydratase (FH) deficie"],"journal":["Cell metabolism"],"pubmed_title":["Comprehensive isotopomer analysis of glutamate and aspartate in small tissue samples."],"pmcid":["PMC10732579"],"funding_grant_id":["P41 GM122698","P50 CA196516","P50 CA070907","R35 CA220449","RM1 GM148766","U2C DK119889","S10 OD028753","F31 CA239330","R01 DK132254"],"pubmed_authors":["Merritt ME","DeBerardinis RJ","Ko B","Pachnis P","Gu W","Bezwada D","Martin-Sandoval M","Cai L","Cai F","Yang C","Do D","Wu Z","Kelekar S","Mathews TP","Brooks B","Malloy CR","Margulis V","Mahar R","Chang MC","Vu HS","Zacharias LG","Oaxaca KC","Jin ES"],"additional_accession":[]},"is_claimable":false,"name":"Comprehensive isotopomer analysis of glutamate and aspartate in small tissue samples.","description":"Stable isotopes are powerful tools to assess metabolism. <sup>13</sup>C labeling is detected using nuclear magnetic resonance (NMR) spectroscopy or mass spectrometry (MS). MS has excellent sensitivity but generally cannot discriminate among different <sup>13</sup>C positions (isotopomers), whereas NMR is less sensitive but reports some isotopomers. Here, we develop an MS method that reports all 16 aspartate and 32 glutamate isotopomers while requiring less than 1% of the sample used for NMR. This method discriminates between pathways that result in the same number of <sup>13</sup>C labels in aspartate and glutamate, providing enhanced specificity over conventional MS. We demonstrate regional metabolic heterogeneity within human tumors, document the impact of fumarate hydratase (FH) deficie","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Oct","modification":"2026-04-12T23:18:22.75Z","creation":"2025-02-19T01:18:31.517Z"},"accession":"S-EPMC10732579","cross_references":{"pubmed":["37611583"],"doi":["10.1016/j.cmet.2023.07.013"]}}