{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11"],"submitter":["Hu M"],"pubmed_abstract":["This study aims to investigate the regulatory mechanism of choline (CH) on triglyceride (TG) synthesis in cows, with a specific focus on its potential association with high milk fat percentage in the gut of the Zhongdian yak. By employing combined metagenomics and metabolomics analysis, we establish a correlation between CH and milk fat production in yaks. Bovine mammary epithelial cells (MAC-T) were exposed to varying CH concentrations, and after 24 h, we analyzed the expression levels of key proteins (membrane glycoprotein CD36 (CD36); adipose differentiation-related protein (ADFP); and ubiquintin (UB)), cellular TG content, lipid droplets, and cell vitality. Additionally, we evaluated the genes potentially related to the CH-mediated regulation of TG synthesis using real-time qPCR. CH at"],"journal":["PeerJ"],"pagination":["e16611"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10740596"],"repository":["biostudies-literature"],"pubmed_title":["Choline regulation of triglycerides synthesis through ubiquintination pathway in MAC-T cells."],"pmcid":["PMC10740596"],"pubmed_authors":["Liu L","Hu M"],"additional_accession":[]},"is_claimable":false,"name":"Choline regulation of triglycerides synthesis through ubiquintination pathway in MAC-T cells.","description":"This study aims to investigate the regulatory mechanism of choline (CH) on triglyceride (TG) synthesis in cows, with a specific focus on its potential association with high milk fat percentage in the gut of the Zhongdian yak. By employing combined metagenomics and metabolomics analysis, we establish a correlation between CH and milk fat production in yaks. Bovine mammary epithelial cells (MAC-T) were exposed to varying CH concentrations, and after 24 h, we analyzed the expression levels of key proteins (membrane glycoprotein CD36 (CD36); adipose differentiation-related protein (ADFP); and ubiquintin (UB)), cellular TG content, lipid droplets, and cell vitality. Additionally, we evaluated the genes potentially related to the CH-mediated regulation of TG synthesis using real-time qPCR. CH at","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2025-04-26T07:49:43.874Z","creation":"2025-04-06T12:29:50.183Z"},"accession":"S-EPMC10740596","cross_references":{"pubmed":["38144203"],"doi":["10.7717/peerj.16611"]}}