biostudies-literatureWang Gthe financial support from the National Natural Science Foundation of Chinathe Natural Science Foundation of Anhui ProvinceNatural Science Foundation of Anhui ProvinceNational Natural Science Foundation of ChinaKey Scientific Research Project in Colleges and the Universities of Anhui Provincethe Key Scientific Research Project in Colleges and the Universities of Anhui Province631https://www.ebi.ac.uk/biostudies/studies/S-EPMC10745014biostudies-literatureUnknown21(12)Six previously undescribed cytosporone derivatives (phomotones A-E (<b>1</b>-<b>5</b>) and phomotone F (<b>13</b>)), two new spiro-alkanol phombistenes A-B (<b>14</b>-<b>15</b>), and seven known analogs (<b>6</b>-<b>12</b>) were isolated from the mangrove endophytic fungus <i>Phomopsis</i> sp. QYM-13. The structures of these compounds were elucidated using spectroscopic data analysis, electronic circular dichroism (ECD), and ¹³C NMR calculations. Compound <b>14</b> features an unprecedented 1,6-dioxaspiro[4.5]decane ring system. All isolates were evaluated for their inhibitory effect on nitric oxide (NO) in LPS-induced RAW264.7 cells. The results showed that compounds <b>1</b>, <b>6</b>, <b>8,</b> and <b>11</b> exhibited potent bioactivities by comparing with positive control. TMarine drugsCytosporones with Anti-Inflammatory Activities from the Mangrove Endophytic Fungus <i>Phomopsis</i> sp. QYM-13.PMC10745014U20A2001422761142308085QH3022022AH050706U20A2001, 42276114Li ZZhu JShe ZWang GChen YYuan YfalseCytosporones with Anti-Inflammatory Activities from the Mangrove Endophytic Fungus <i>Phomopsis</i> sp. QYM-13.Six previously undescribed cytosporone derivatives (phomotones A-E (<b>1</b>-<b>5</b>) and phomotone F (<b>13</b>)), two new spiro-alkanol phombistenes A-B (<b>14</b>-<b>15</b>), and seven known analogs (<b>6</b>-<b>12</b>) were isolated from the mangrove endophytic fungus <i>Phomopsis</i> sp. QYM-13. The structures of these compounds were elucidated using spectroscopic data analysis, electronic circular dichroism (ECD), and ¹³C NMR calculations. Compound <b>14</b> features an unprecedented 1,6-dioxaspiro[4.5]decane ring system. All isolates were evaluated for their inhibitory effect on nitric oxide (NO) in LPS-induced RAW264.7 cells. The results showed that compounds <b>1</b>, <b>6</b>, <b>8,</b> and <b>11</b> exhibited potent bioactivities by comparing with positive control. T2023-01-01T00:00:00Z2023 Dec2026-05-14T03:19:29.263Z2025-04-06T16:28:05.496ZS-EPMC107450143813295210.3390/md21120631