biostudies-literatureUnknown9(12)Qin PThe Gram-negative bacterium <i>Pseudomonas plecoglossicida</i> has caused visceral granulomas disease in several farmed fish species, including large yellow croaker (<i>Larimichthys crocea</i>), which results in severe economic losses. Type III secretion systems (T3SS) are protein secretion and translocation nanomachines widely employed by many Gram-negative bacterial pathogens for infection and pathogenicity. However, the exact role of T3SS in the pathogenesis of <i>P. plecoglossicida</i> infection is still unclear. In this study, a T3SS translocators deletion strain (△<i>popBD</i>) of <i>P. plecoglossicida</i> was constructed to investigate the function of T3SS. Then comparative secretome analysis of the <i>P. plecoglossicida</i> wild-type (WT) and △<i>popBD</i> mutant strains was conducHeliyone22669https://www.ebi.ac.uk/biostudies/studies/S-EPMC10746435biostudies-literatureComparative secretome analysis reveals cross-talk between type III secretion system and flagella assembly in <i>Pseudomonas plecoglossicida</i>.PMC10746435Qin PLuan YYang JLi YShao GMunang'andu HMChen XWu TfalseComparative secretome analysis reveals cross-talk between type III secretion system and flagella assembly in <i>Pseudomonas plecoglossicida</i>.The Gram-negative bacterium <i>Pseudomonas plecoglossicida</i> has caused visceral granulomas disease in several farmed fish species, including large yellow croaker (<i>Larimichthys crocea</i>), which results in severe economic losses. Type III secretion systems (T3SS) are protein secretion and translocation nanomachines widely employed by many Gram-negative bacterial pathogens for infection and pathogenicity. However, the exact role of T3SS in the pathogenesis of <i>P. plecoglossicida</i> infection is still unclear. In this study, a T3SS translocators deletion strain (△<i>popBD</i>) of <i>P. plecoglossicida</i> was constructed to investigate the function of T3SS. Then comparative secretome analysis of the <i>P. plecoglossicida</i> wild-type (WT) and △<i>popBD</i> mutant strains was conduc2023-01-01T00:00:00Z2023 Dec2026-04-08T15:20:14.42Z2025-02-19T01:46:06.375ZS-EPMC107464353814433610.1016/j.heliyon.2023.e22669