{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(1)"],"submitter":["Li J"],"pubmed_abstract":["<h4>Objective</h4>To establish a juvenile mouse asthma model by postnatal hyperoxia exposure combined with early ovalbumin (OVA) sensitization.<h4>Methods</h4>Female C57BL/6J newborn mice were exposed to hyperoxia (95 % O<sub>2</sub>) from postnatal day-1 (PND1) to PND7; intraperitoneally injected with OVA suspension on PND21, PND28; and stimulated by nebulized inhalation of 1 % OVA from PND36 to PND42. Within 48 h of the last challenge, we observed their activity performance and evaluated airway responsiveness (AHR). All mice were executed at PND44. Female (n = 32) were divided into four groups as follows: room air（RA）+phosphate-buffered saline (PBS) group; O<sub>2</sub> (hyperoxia, 95 % O<sub>2</sub>) + PBS group; RA + OVA group; O<sub>2</sub>+OVA group. We obtained the serum, bronchoalv"],"journal":["Heliyon"],"pagination":["e23291"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10750071"],"repository":["biostudies-literature"],"pubmed_title":["Establishment of a juvenile mouse asthma model induced by postnatal hyperoxia exposure combined with early OVA sensitization."],"pmcid":["PMC10750071"],"pubmed_authors":["Cao L","Tian Z","Li J","Yu B","Ma M","Zhang Y","Wu R","Zhu H","Bao T"],"additional_accession":[]},"is_claimable":false,"name":"Establishment of a juvenile mouse asthma model induced by postnatal hyperoxia exposure combined with early OVA sensitization.","description":"<h4>Objective</h4>To establish a juvenile mouse asthma model by postnatal hyperoxia exposure combined with early ovalbumin (OVA) sensitization.<h4>Methods</h4>Female C57BL/6J newborn mice were exposed to hyperoxia (95 % O<sub>2</sub>) from postnatal day-1 (PND1) to PND7; intraperitoneally injected with OVA suspension on PND21, PND28; and stimulated by nebulized inhalation of 1 % OVA from PND36 to PND42. Within 48 h of the last challenge, we observed their activity performance and evaluated airway responsiveness (AHR). All mice were executed at PND44. Female (n = 32) were divided into four groups as follows: room air（RA）+phosphate-buffered saline (PBS) group; O<sub>2</sub> (hyperoxia, 95 % O<sub>2</sub>) + PBS group; RA + OVA group; O<sub>2</sub>+OVA group. We obtained the serum, bronchoalv","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jan","modification":"2025-04-05T15:53:47.132Z","creation":"2025-04-05T15:53:47.132Z"},"accession":"S-EPMC10750071","cross_references":{"pubmed":["38148813"],"doi":["10.1016/j.heliyon.2023.e23291"]}}