biostudies-literatureUnknown10(1)Li J<h4>Objective</h4>To establish a juvenile mouse asthma model by postnatal hyperoxia exposure combined with early ovalbumin (OVA) sensitization.<h4>Methods</h4>Female C57BL/6J newborn mice were exposed to hyperoxia (95 % O₂) from postnatal day-1 (PND1) to PND7; intraperitoneally injected with OVA suspension on PND21, PND28; and stimulated by nebulized inhalation of 1 % OVA from PND36 to PND42. Within 48 h of the last challenge, we observed their activity performance and evaluated airway responsiveness (AHR). All mice were executed at PND44. Female (n = 32) were divided into four groups as follows: room air（RA）+phosphate-buffered saline (PBS) group; O₂ (hyperoxia, 95 % O₂) + PBS group; RA + OVA group; O₂+OVA group. We obtained the serum, bronchoalvHeliyone23291https://www.ebi.ac.uk/biostudies/studies/S-EPMC10750071biostudies-literatureEstablishment of a juvenile mouse asthma model induced by postnatal hyperoxia exposure combined with early OVA sensitization.PMC10750071Cao LTian ZLi JYu BMa MZhang YWu RZhu HBao TfalseEstablishment of a juvenile mouse asthma model induced by postnatal hyperoxia exposure combined with early OVA sensitization.<h4>Objective</h4>To establish a juvenile mouse asthma model by postnatal hyperoxia exposure combined with early ovalbumin (OVA) sensitization.<h4>Methods</h4>Female C57BL/6J newborn mice were exposed to hyperoxia (95 % O₂) from postnatal day-1 (PND1) to PND7; intraperitoneally injected with OVA suspension on PND21, PND28; and stimulated by nebulized inhalation of 1 % OVA from PND36 to PND42. Within 48 h of the last challenge, we observed their activity performance and evaluated airway responsiveness (AHR). All mice were executed at PND44. Female (n = 32) were divided into four groups as follows: room air（RA）+phosphate-buffered saline (PBS) group; O₂ (hyperoxia, 95 % O₂) + PBS group; RA + OVA group; O₂+OVA group. We obtained the serum, bronchoalv2024-01-01T00:00:00Z2024 Jan2025-04-05T15:53:47.132Z2025-04-05T15:53:47.132ZS-EPMC107500713814881310.1016/j.heliyon.2023.e23291