biostudies-literatureKobayashi YMinistry of Education, Culture, Sports, Science and TechnologyTorii Pharmaceutical100198https://www.ebi.ac.uk/biostudies/studies/S-EPMC10753085biostudies-literatureUnknown3(1)<h4>Background</h4>In Japan, pollinosis caused by the Japanese cypress (JCy) <i>Chamaecyparis obtusa</i> is among the very common seasonal allergies. In JCy pollinosis, Cha o 1 is the first major allergen, and Cha o 2 is the second major allergen. Recently, Cha o 3 was identified as a new JCy pollen allergen in JCy pollinosis. However, the relative contribution of Cha o 3 to JCy pollinosis compared with that of Cha o 1 and that of Cha o 2 has not been fully elucidated.<h4>Objective</h4>This study aimed to clarify the allergenicity of Cha o 3 compared with that of Cha o 1 and Cha o 2 in JCy pollinosis.<h4>Methods</h4>We recruited 27 patients with JCy pollinosis and performed the basophil activation test (BAT) with native (n) Cha o 1, Cha o 2, and Cha o 3 purified from JCy pollen. In addition, we a performed JCy-specific IgE suppression test.<h4>Results</h4>In the BAT, 26 of 27 patients (96%) and 18 of 27 patients (67%) showed positive basophil activation in response to n Cha o 1 and n Cha o 2, respectively, as judged by CD203c expression. Little CD203c expression in response to n Cha o 3 was seen. The presence of n Cha o 3 marginally reduced the titer levels of JCy-specific IgE.<h4>Conclusion</h4>Cha o 3 showed little ability to activate basophils and suppress JCy-specific IgE titers compared with Cha o 1 or Cha o 2 in patients with JCy pollinosis. Thus, Cha o 3 may not be a major allergen in JCy pollinosis.The journal of allergy and clinical immunology. GlobalCha o 3, a cypress pollen allergen, does not activate basophils in Japanese cypress pollinosis.PMC1075308522K19427Nagasaka YSakurai DNakamura YKobayashi YTateno MIshimaru KOhashi-Doi KSuzuki KNakao AfalseCha o 3, a cypress pollen allergen, does not activate basophils in Japanese cypress pollinosis.<h4>Background</h4>In Japan, pollinosis caused by the Japanese cypress (JCy) <i>Chamaecyparis obtusa</i> is among the very common seasonal allergies. In JCy pollinosis, Cha o 1 is the first major allergen, and Cha o 2 is the second major allergen. Recently, Cha o 3 was identified as a new JCy pollen allergen in JCy pollinosis. However, the relative contribution of Cha o 3 to JCy pollinosis compared with that of Cha o 1 and that of Cha o 2 has not been fully elucidated.<h4>Objective</h4>This study aimed to clarify the allergenicity of Cha o 3 compared with that of Cha o 1 and Cha o 2 in JCy pollinosis.<h4>Methods</h4>We recruited 27 patients with JCy pollinosis and performed the basophil activation test (BAT) with native (n) Cha o 1, Cha o 2, and Cha o 3 purified from JCy pollen. In addition, we a performed JCy-specific IgE suppression test.<h4>Results</h4>In the BAT, 26 of 27 patients (96%) and 18 of 27 patients (67%) showed positive basophil activation in response to n Cha o 1 and n Cha o 2, respectively, as judged by CD203c expression. Little CD203c expression in response to n Cha o 3 was seen. The presence of n Cha o 3 marginally reduced the titer levels of JCy-specific IgE.<h4>Conclusion</h4>Cha o 3 showed little ability to activate basophils and suppress JCy-specific IgE titers compared with Cha o 1 or Cha o 2 in patients with JCy pollinosis. Thus, Cha o 3 may not be a major allergen in JCy pollinosis.2024-01-01T00:00:00Z2024 Feb2024-10-18T04:46:17.169Z2024-10-18T04:46:17.169ZS-EPMC107530853815586110.1016/j.jacig.2023.100198