{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["55(12)"],"submitter":["Zhu Y"],"pubmed_abstract":["Krüppel-like zinc-finger transcription factor 5 (KLF5) is a vital regulator of breast cancer (BC) onset and progression. The mechanism by which KLF5 regulates BC is still not clearly known. In this study, bioinformatics analysis shows that BC-affected individuals with elevated KLF5 expression levels have poor clinical outcomes. We further verify that miR-145-5p regulated KLF5 expression to promote cell apoptosis and inhibit cell proliferation in BC via dual-luciferase reporter assay, western blot analysis, qRT-PCR, CCK-8 assay and cell apoptosis assay. In addition, based on bioinformatics analysis, the binding of ENST00000422059 with miR-145-5p is confirmed by dual-luciferase reporter assay. Subsequently, FISH, western blot analysis, qRT-PCR, CCK-8 and cell apoptosis assays verified that E"],"journal":["Acta biochimica et biophysica Sinica"],"pagination":["1892-1901"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10753370"],"repository":["biostudies-literature"],"pubmed_title":["lncRNA ENST00000422059 promotes cell proliferation and inhibits cell apoptosis in breast cancer by regulating the miR-145-5p/KLF5 axis."],"pmcid":["PMC10753370"],"pubmed_authors":["Jiang Y","Li Y","Zhu Y","Ren J","Zhang Y","Wang Y","Xu J","Wu X","Tan Q"],"additional_accession":[]},"is_claimable":false,"name":"lncRNA ENST00000422059 promotes cell proliferation and inhibits cell apoptosis in breast cancer by regulating the miR-145-5p/KLF5 axis.","description":"Krüppel-like zinc-finger transcription factor 5 (KLF5) is a vital regulator of breast cancer (BC) onset and progression. The mechanism by which KLF5 regulates BC is still not clearly known. In this study, bioinformatics analysis shows that BC-affected individuals with elevated KLF5 expression levels have poor clinical outcomes. We further verify that miR-145-5p regulated KLF5 expression to promote cell apoptosis and inhibit cell proliferation in BC via dual-luciferase reporter assay, western blot analysis, qRT-PCR, CCK-8 assay and cell apoptosis assay. In addition, based on bioinformatics analysis, the binding of ENST00000422059 with miR-145-5p is confirmed by dual-luciferase reporter assay. Subsequently, FISH, western blot analysis, qRT-PCR, CCK-8 and cell apoptosis assays verified that E","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2025-04-26T07:00:29.48Z","creation":"2025-04-06T12:04:46.5Z"},"accession":"S-EPMC10753370","cross_references":{"pubmed":["37997376"],"doi":["10.3724/abbs.2023226"]}}