biostudies-literatureSohmen BBundesministerium für Bildung und ForschungEuropean Research CouncilDeutsche Forschungsgemeinschafte2304262https://www.ebi.ac.uk/biostudies/studies/S-EPMC10754087biostudies-literatureUnknown10(36)Protein dynamics have been investigated on a wide range of time scales. Nano- and picosecond dynamics have been assigned to local fluctuations, while slower dynamics have been attributed to larger conformational changes. However, it is largely unknown how fast (local) fluctuations can lead to slow global (allosteric) changes. Here, fast molecule-spanning dynamics on the 100 to 200 ns time scale in the heat shock protein 90 (Hsp90) are shown. Global real-space movements are assigned to dynamic modes on this time scale, which is possible by a combination of single-molecule fluorescence, quasi-elastic neutron scattering and all-atom molecular dynamics (MD) simulations. The time scale of these dynamic modes depends on the conformational state of the Hsp90 dimer. In addition, the dynamic modes Advanced science (Weinheim, Baden-Wurttemberg, Germany)The Onset of Molecule-Spanning Dynamics in Heat Shock Protein Hsp90.PMC1075408768189140322270INST 37/935‐1 FUGGINST 37/935-1 FUGG43194560FKZ 05K19VTBSohmen BSchreiber FWolf SBeck CGrimaldo MRoosen-Runge FHoffmann INuesch MHugel TSeydel THermann BFrank VfalseThe Onset of Molecule-Spanning Dynamics in Heat Shock Protein Hsp90.Protein dynamics have been investigated on a wide range of time scales. Nano- and picosecond dynamics have been assigned to local fluctuations, while slower dynamics have been attributed to larger conformational changes. However, it is largely unknown how fast (local) fluctuations can lead to slow global (allosteric) changes. Here, fast molecule-spanning dynamics on the 100 to 200 ns time scale in the heat shock protein 90 (Hsp90) are shown. Global real-space movements are assigned to dynamic modes on this time scale, which is possible by a combination of single-molecule fluorescence, quasi-elastic neutron scattering and all-atom molecular dynamics (MD) simulations. The time scale of these dynamic modes depends on the conformational state of the Hsp90 dimer. In addition, the dynamic modes 2023-01-01T00:00:00Z2023 Dec2026-07-15T13:59:36.557Z2025-04-04T10:30:21.19ZS-EPMC107540873798488710.1002/advs.202304262