biostudies-literatureSilva TPFundação para a Ciência e a Tecnologia725-742https://www.ebi.ac.uk/biostudies/studies/S-EPMC10758933biostudies-literatureUnknown14(1)Biodesulfurization is a biotechnological process that uses microorganisms as biocatalysts to actively remove sulfur from fuels. It has the potential to be cleaner and more efficient than the current industrial process, however several bottlenecks have prevented its implementation. Additionally, most works propose models based on direct cultivation on fuel, or batch production of biocatalysts followed by a processing step before application to batch biodesulfurization, which are difficult to replicate at a larger scale. Thus, there is a need for a model that can be adapted to a refining process, where fuel is being continuously produced to meet consumer needs. The main goal of this work was to develop the first bench-scale continuous biodesulfurization system that integrates biocatalyst proRSC advancesStreamlining the biodesulfurization process: development of an integrated continuous system prototype using <i>Gordonia alkanivorans</i> strain 1B.PMC10758933SFRH/BD/104977/2014PTDC/EAM-AMB/30975/2017 – Project GreenFuelTavares JParadela FRoseiro JCAlves LSilva TPCrujeira TPaixao SMfalseStreamlining the biodesulfurization process: development of an integrated continuous system prototype using <i>Gordonia alkanivorans</i> strain 1B.Biodesulfurization is a biotechnological process that uses microorganisms as biocatalysts to actively remove sulfur from fuels. It has the potential to be cleaner and more efficient than the current industrial process, however several bottlenecks have prevented its implementation. Additionally, most works propose models based on direct cultivation on fuel, or batch production of biocatalysts followed by a processing step before application to batch biodesulfurization, which are difficult to replicate at a larger scale. Thus, there is a need for a model that can be adapted to a refining process, where fuel is being continuously produced to meet consumer needs. The main goal of this work was to develop the first bench-scale continuous biodesulfurization system that integrates biocatalyst pro2024-01-01T00:00:00Z2024 Jan2026-06-03T13:30:20.269Z2025-05-29T16:36:37.234ZS-EPMC107589333817359610.1039/d3ra07405f