biostudies-literatureLin Ythe Natural Science Foundation of Fujian Provincethe Young and Middle-aged Backbone Research Fund of Fujian Provincial Health Care CommissionNational Natural Science Foundation of ChinaStartup Fund for scientific research, Fujian Medical UniversityFujian Provincial Medical Science and Technology Innovation Joint Fund Project1https://www.ebi.ac.uk/biostudies/studies/S-EPMC10759737biostudies-literatureUnknown43(1)<h4>Background</h4>Ceramide metabolism is crucial in the progress of brain metastasis (BM). However, it remains unexplored whether targeting ceramide metabolism may arrest BM.<h4>Methods</h4>RNA sequencing was applied to screen different genes in primary and metastatic foci and whole-exome sequencing (WES) to seek crucial abnormal pathway in BM + and BM-patients. Cellular arrays were applied to analyze the permeability of blood-brain barrier (BBB) and the activation or inhibition of pathway. Database and Co-Immunoprecipitation (Co-IP) assay were adopted to verify the protein-protein interaction. Xenograft and zebrafish model were further employed to verify the cellular results.<h4>Results</h4>RNA sequencing and WES reported the involvement of RPTOR and ceramide metabolism in BM progress. RJournal of experimental & clinical cancer research : CRRPTOR blockade suppresses brain metastases of NSCLC by interfering the ceramide metabolism via hijacking YY1 binding.PMC107597372020J0111062018QH11302020Y9023820024572019-ZQNB-12020GGA0102013J011177Li HPan JXu NShe PLin YChen YChen SNiu GWu YZhang QTu XLin MfalseRPTOR blockade suppresses brain metastases of NSCLC by interfering the ceramide metabolism via hijacking YY1 binding.<h4>Background</h4>Ceramide metabolism is crucial in the progress of brain metastasis (BM). However, it remains unexplored whether targeting ceramide metabolism may arrest BM.<h4>Methods</h4>RNA sequencing was applied to screen different genes in primary and metastatic foci and whole-exome sequencing (WES) to seek crucial abnormal pathway in BM + and BM-patients. Cellular arrays were applied to analyze the permeability of blood-brain barrier (BBB) and the activation or inhibition of pathway. Database and Co-Immunoprecipitation (Co-IP) assay were adopted to verify the protein-protein interaction. Xenograft and zebrafish model were further employed to verify the cellular results.<h4>Results</h4>RNA sequencing and WES reported the involvement of RPTOR and ceramide metabolism in BM progress. R2024-01-01T00:00:00Z2024 Jan2025-04-04T14:43:21.735Z2024-11-20T22:55:06.25ZS-EPMC107597373816389010.1186/s13046-023-02874-z