biostudies-literatureUnknownSorensen Turpin CGNHGRI NIH HHSNIGMS NIH HHSNIH HHSSeparase is a key regulator of the metaphase to anaphase transition with multiple functions. Separase cleaves cohesin to allow chromosome segregation and localizes to vesicles to promote exocytosis in mid-anaphase. The anaphase promoting complex/cyclosome (APC/C) activates separase by ubiquitinating its inhibitory chaperone, securin, triggering its degradation. How this pathway controls the exocytic function of separase has not been investigated. During meiosis I, securin is degraded over several minutes, while separase rapidly relocalizes from kinetochore structures at the spindle and cortex to sites of action on chromosomes and vesicles at anaphase onset. The loss of cohesin coincides with the relocalization of separase to the chromosome midbivalent at anaphase onset. APC/C depletion prebioRxiv : the preprint server for biology2023.12.12.571338https://www.ebi.ac.uk/biostudies/studies/S-EPMC10760073biostudies-literatureSecurin Regulates the Spatiotemporal Dynamics of Separase.PMC10760073R15 GM143731P40 OD010440R01 GM114471R15 GM117548U41 HG002223LaForest MSorensen Turpin CGKlebanow LUSeverson AFBembenek JNSloan DMitchell DfalseSecurin Regulates the Spatiotemporal Dynamics of Separase.Separase is a key regulator of the metaphase to anaphase transition with multiple functions. Separase cleaves cohesin to allow chromosome segregation and localizes to vesicles to promote exocytosis in mid-anaphase. The anaphase promoting complex/cyclosome (APC/C) activates separase by ubiquitinating its inhibitory chaperone, securin, triggering its degradation. How this pathway controls the exocytic function of separase has not been investigated. During meiosis I, securin is degraded over several minutes, while separase rapidly relocalizes from kinetochore structures at the spindle and cortex to sites of action on chromosomes and vesicles at anaphase onset. The loss of cohesin coincides with the relocalization of separase to the chromosome midbivalent at anaphase onset. APC/C depletion pre2023-01-01T00:00:00Z2023 Dec2026-04-08T04:03:37.32Z2025-02-19T01:21:05.178ZS-EPMC107600733816840210.1101/2023.12.12.571338