{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Russo M"],"funding":["European Research Council","Consortium ENHPATHY","Italian Association for Research on Cancer","Marie Sklodowska-Curie Action","Horizon 2020 European Training Network"],"pagination":["1017-1040"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10760643"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["37(21-24)"],"pubmed_abstract":["Transcription termination pathways mitigate the detrimental consequences of unscheduled promiscuous initiation occurring at hundreds of thousands of genomic <i>cis</i>-regulatory elements. The Restrictor complex, composed of the Pol II-interacting protein WDR82 and the RNA-binding protein ZC3H4, suppresses processive transcription at thousands of extragenic sites in mammalian genomes. Restrictor-driven termination does not involve nascent RNA cleavage, and its interplay with other termination machineries is unclear. Here we show that efficient termination at Restrictor-controlled extragenic transcription units involves the recruitment of the protein phosphatase 1 (PP1) regulatory subunit PNUTS, a negative regulator of the SPT5 elongation factor, and Symplekin, a protein associated with RNA"],"journal":["Genes & development"],"pubmed_title":["Restrictor synergizes with Symplekin and PNUTS to terminate extragenic transcription."],"pmcid":["PMC10760643"],"funding_grant_id":["692789","860002"],"pubmed_authors":["Polletti S","Russo M","Polizzese D","Piccolo V","Mandana GM","Bedin F","Rodighiero S","Natoli G","Borriero C","Marenda M","Michieletto D","Cuomo A","Prosperini E"],"additional_accession":[]},"is_claimable":false,"name":"Restrictor synergizes with Symplekin and PNUTS to terminate extragenic transcription.","description":"Transcription termination pathways mitigate the detrimental consequences of unscheduled promiscuous initiation occurring at hundreds of thousands of genomic <i>cis</i>-regulatory elements. The Restrictor complex, composed of the Pol II-interacting protein WDR82 and the RNA-binding protein ZC3H4, suppresses processive transcription at thousands of extragenic sites in mammalian genomes. Restrictor-driven termination does not involve nascent RNA cleavage, and its interplay with other termination machineries is unclear. Here we show that efficient termination at Restrictor-controlled extragenic transcription units involves the recruitment of the protein phosphatase 1 (PP1) regulatory subunit PNUTS, a negative regulator of the SPT5 elongation factor, and Symplekin, a protein associated with RNA","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Dec","modification":"2026-06-01T23:37:07.166Z","creation":"2026-05-23T03:08:04.016Z"},"accession":"S-EPMC10760643","cross_references":{"pubmed":["38092518"],"doi":["10.1101/gad.351057.123"]}}