{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Roh TH"],"funding":["Ministry of Science and ICT","Yonsei University College of Medicine","Soonchunhyang University","National Research Foundation of Korea"],"pagination":["739-749"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10765002"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["38(6)"],"pubmed_abstract":["Backgruound
Phospholipase C-γ (PLC-γ) plays a crucial role in immune responses and is related to the pathogenesis of various inflammatory disorders. In this study, we investigated the role of PLC-γ and the therapeutic effect of the PLC-specific inhibitor U73122 using orbital fibroblasts from patients with Graves' orbitopathy (GO).Methods
The expression of phospholipase C gamma 1 (PLCG1) and phospholipase C gamma 2 (PLCG2) was evaluated using polymerase chain reaction in GO and normal orbital tissues/fibroblasts. The primary cultures of orbital fibroblasts were treated with non-toxic concentrations of U73122 with or without interleukin (IL)-1β to determine its therapeutic efficacy. The proinflammatory cytokine levels and activation of downstream signaling molecules were dete"],"journal":["Endocrinology and metabolism (Seoul, Korea)"],"pubmed_title":["Phospholipase C-γ as a Potential Therapeutic Target for Graves' Orbitopathy."],"pmcid":["PMC10765002"],"funding_grant_id":["2021R1F1A1046652"],"pubmed_authors":["Roh TH","Kikkawa DO","Ko JS","Jang SY","Yoon JS","Chae MK"],"additional_accession":[]},"is_claimable":false,"name":"Phospholipase C-γ as a Potential Therapeutic Target for Graves' Orbitopathy.","description":"Backgruound
Phospholipase C-γ (PLC-γ) plays a crucial role in immune responses and is related to the pathogenesis of various inflammatory disorders. In this study, we investigated the role of PLC-γ and the therapeutic effect of the PLC-specific inhibitor U73122 using orbital fibroblasts from patients with Graves' orbitopathy (GO).Methods
The expression of phospholipase C gamma 1 (PLCG1) and phospholipase C gamma 2 (PLCG2) was evaluated using polymerase chain reaction in GO and normal orbital tissues/fibroblasts. The primary cultures of orbital fibroblasts were treated with non-toxic concentrations of U73122 with or without interleukin (IL)-1β to determine its therapeutic efficacy. The proinflammatory cytokine levels and activation of downstream signaling molecules were dete","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Dec","modification":"2026-07-14T21:23:37.869Z","creation":"2025-02-19T01:21:02.148Z"},"accession":"S-EPMC10765002","cross_references":{"pubmed":["37989267"],"doi":["10.3803/EnM.2023.1780"]}}