biostudies-literatureRoh THMinistry of Science and ICTYonsei University College of MedicineSoonchunhyang UniversityNational Research Foundation of Korea739-749https://www.ebi.ac.uk/biostudies/studies/S-EPMC10765002biostudies-literatureUnknown38(6)<h4>Backgruound</h4>Phospholipase C-γ (PLC-γ) plays a crucial role in immune responses and is related to the pathogenesis of various inflammatory disorders. In this study, we investigated the role of PLC-γ and the therapeutic effect of the PLC-specific inhibitor U73122 using orbital fibroblasts from patients with Graves' orbitopathy (GO).<h4>Methods</h4>The expression of phospholipase C gamma 1 (PLCG1) and phospholipase C gamma 2 (PLCG2) was evaluated using polymerase chain reaction in GO and normal orbital tissues/fibroblasts. The primary cultures of orbital fibroblasts were treated with non-toxic concentrations of U73122 with or without interleukin (IL)-1β to determine its therapeutic efficacy. The proinflammatory cytokine levels and activation of downstream signaling molecules were deteEndocrinology and metabolism (Seoul, Korea)Phospholipase C-γ as a Potential Therapeutic Target for Graves' Orbitopathy.PMC107650022021R1F1A1046652Roh THKikkawa DOKo JSJang SYYoon JSChae MKfalsePhospholipase C-γ as a Potential Therapeutic Target for Graves' Orbitopathy.<h4>Backgruound</h4>Phospholipase C-γ (PLC-γ) plays a crucial role in immune responses and is related to the pathogenesis of various inflammatory disorders. In this study, we investigated the role of PLC-γ and the therapeutic effect of the PLC-specific inhibitor U73122 using orbital fibroblasts from patients with Graves' orbitopathy (GO).<h4>Methods</h4>The expression of phospholipase C gamma 1 (PLCG1) and phospholipase C gamma 2 (PLCG2) was evaluated using polymerase chain reaction in GO and normal orbital tissues/fibroblasts. The primary cultures of orbital fibroblasts were treated with non-toxic concentrations of U73122 with or without interleukin (IL)-1β to determine its therapeutic efficacy. The proinflammatory cytokine levels and activation of downstream signaling molecules were dete2023-01-01T00:00:00Z2023 Dec2026-07-14T21:23:37.869Z2025-02-19T01:21:02.148ZS-EPMC107650023798926710.3803/EnM.2023.1780