{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ward CK"],"funding":["NIAID NIH HHS"],"pagination":["6013-22"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC107678"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["180(22)"],"pubmed_abstract":["We have identified two extremely large open reading frames (ORFs) in Haemophilus ducreyi 35000, lspA1 and lspA2, each of which encodes a predicted protein product whose N-terminal half is approximately 43% similar to the N-terminal half of Bordetella pertussis filamentous hemagglutinin (FhaB). To the best of our knowledge, lspA1 (12,500 nucleotides [nt]) and lspA2 (14,800 nt) are among the largest prokaryotic ORFs identified to date. The predicted proteins, LspA1 and LspA2, are 86% identical overall to each other and also have limited amino acid sequence similarity at their N termini to other secreted bacterial proteins, including certain hemolysins. Southern blot analysis indicated that lspA1 and lspA2 sequences were present in 15 other geographically diverse H. ducreyi strains. Reverse t"],"journal":["Journal of bacteriology"],"pubmed_title":["Haemophilus ducreyi secretes a filamentous hemagglutinin-like protein."],"pmcid":["PMC107678"],"funding_grant_id":["F32-AI09845","AI32011"],"pubmed_authors":["Cope LD","Hansen EJ","Ward CK","Lumbley SR","Latimer JL"],"additional_accession":[]},"is_claimable":false,"name":"Haemophilus ducreyi secretes a filamentous hemagglutinin-like protein.","description":"We have identified two extremely large open reading frames (ORFs) in Haemophilus ducreyi 35000, lspA1 and lspA2, each of which encodes a predicted protein product whose N-terminal half is approximately 43% similar to the N-terminal half of Bordetella pertussis filamentous hemagglutinin (FhaB). To the best of our knowledge, lspA1 (12,500 nucleotides [nt]) and lspA2 (14,800 nt) are among the largest prokaryotic ORFs identified to date. The predicted proteins, LspA1 and LspA2, are 86% identical overall to each other and also have limited amino acid sequence similarity at their N termini to other secreted bacterial proteins, including certain hemolysins. Southern blot analysis indicated that lspA1 and lspA2 sequences were present in 15 other geographically diverse H. ducreyi strains. Reverse t","dates":{"release":"1998-01-01T00:00:00Z","publication":"1998 Nov","modification":"2025-04-04T09:53:14.251Z","creation":"2019-03-27T00:16:58Z"},"accession":"S-EPMC107678","cross_references":{"pubmed":["9811662"],"doi":["10.1128/JB.180.22.6013-6022.1998"]}}