{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Desai JV"],"funding":["National Institute of Allergy and Infectious Diseases","National Institute of Allergy and Infectious Diseases Division of Intramural Research","New Jersey Health Foundation","NIAID NIH HHS","National Institutes of Health"],"pagination":["102781"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10770751"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["5(1)"],"pubmed_abstract":["Myeloid phagocytes are essential for antifungal host defense during systemic candidiasis. Here, we present a protocol for assessing phagocyte-fungal interactions in vivo in the kidney, the primary target organ of the murine systemic candidiasis model. We describe steps for intravital confocal microscopy and flow cytometry. We also detail a kidney tissue dissociation procedure to obtain highly pure functional phagocytes for utilization in downstream ex vivo fungal uptake and killing assays."],"journal":["STAR protocols"],"pubmed_title":["Evaluation of murine renal phagocyte-fungal interactions using intravital confocal microscopy and flow cytometry."],"pmcid":["PMC10770751"],"funding_grant_id":["R00AI141622","PC 177-23","R00 AI141622"],"pubmed_authors":["Lionakis MS","Desai JV"],"additional_accession":[]},"is_claimable":false,"name":"Evaluation of murine renal phagocyte-fungal interactions using intravital confocal microscopy and flow cytometry.","description":"Myeloid phagocytes are essential for antifungal host defense during systemic candidiasis. Here, we present a protocol for assessing phagocyte-fungal interactions in vivo in the kidney, the primary target organ of the murine systemic candidiasis model. We describe steps for intravital confocal microscopy and flow cytometry. We also detail a kidney tissue dissociation procedure to obtain highly pure functional phagocytes for utilization in downstream ex vivo fungal uptake and killing assays.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-06-21T03:11:31.64Z","creation":"2025-04-04T21:55:13.605Z"},"accession":"S-EPMC10770751","cross_references":{"pubmed":["38113143"],"doi":["10.1016/j.xpro.2023.102781"]}}