biostudies-literatureDesai JVNational Institute of Allergy and Infectious DiseasesNational Institute of Allergy and Infectious Diseases Division of Intramural ResearchNew Jersey Health FoundationNIAID NIH HHSNational Institutes of Health102781https://www.ebi.ac.uk/biostudies/studies/S-EPMC10770751biostudies-literatureUnknown5(1)Myeloid phagocytes are essential for antifungal host defense during systemic candidiasis. Here, we present a protocol for assessing phagocyte-fungal interactions in vivo in the kidney, the primary target organ of the murine systemic candidiasis model. We describe steps for intravital confocal microscopy and flow cytometry. We also detail a kidney tissue dissociation procedure to obtain highly pure functional phagocytes for utilization in downstream ex vivo fungal uptake and killing assays.STAR protocolsEvaluation of murine renal phagocyte-fungal interactions using intravital confocal microscopy and flow cytometry.PMC10770751R00AI141622PC 177-23R00 AI141622Lionakis MSDesai JVfalseEvaluation of murine renal phagocyte-fungal interactions using intravital confocal microscopy and flow cytometry.Myeloid phagocytes are essential for antifungal host defense during systemic candidiasis. Here, we present a protocol for assessing phagocyte-fungal interactions in vivo in the kidney, the primary target organ of the murine systemic candidiasis model. We describe steps for intravital confocal microscopy and flow cytometry. We also detail a kidney tissue dissociation procedure to obtain highly pure functional phagocytes for utilization in downstream ex vivo fungal uptake and killing assays.2024-01-01T00:00:00Z2024 Mar2026-06-21T03:11:31.64Z2025-04-04T21:55:13.605ZS-EPMC107707513811314310.1016/j.xpro.2023.102781