biostudies-literatureHe QNational Natural Science Foundation of China24https://www.ebi.ac.uk/biostudies/studies/S-EPMC10773052biostudies-literatureUnknown24(1)<h4>Background</h4>There are accumulating type 2 diabetes patients who have osteoporosis simultaneously. More effective therapeutic strategies should be discovered. Biochanin A (BCA) has been indicated that can play a role in improving metabolic disorders of type 2 diabetes and preventing osteoporosis. But whether BCA can treat type 2 diabetic osteoporosis has not been studied.<h4>Purpose</h4>To investigate if the BCA can protect against type 2 diabetic osteoporosis and clarify the mechanism.<h4>Methods</h4>Micro-CT and histology assays were performed to detect the trabecular bone and analyze the bone histomorphology effect of BCA. CCK-8 assay was performed to detect the toxicity of BCA. TRAcP staining, immunofluorescence and hydroxyapatite resorption assay were used to observe osteoclastsBMC complementary medicine and therapiesBiochanin A abrogates osteoclastogenesis in type 2 diabetic osteoporosis via regulating ROS/MAPK signaling pathway based on integrating molecular docking and experimental validation.PMC10773052NO. 8207446282374470Yang JPan ZChen PZhang NLin YWang HHe QChen CChen BLi MLi SXiao JChen WZeng JfalseBiochanin A abrogates osteoclastogenesis in type 2 diabetic osteoporosis via regulating ROS/MAPK signaling pathway based on integrating molecular docking and experimental validation.<h4>Background</h4>There are accumulating type 2 diabetes patients who have osteoporosis simultaneously. More effective therapeutic strategies should be discovered. Biochanin A (BCA) has been indicated that can play a role in improving metabolic disorders of type 2 diabetes and preventing osteoporosis. But whether BCA can treat type 2 diabetic osteoporosis has not been studied.<h4>Purpose</h4>To investigate if the BCA can protect against type 2 diabetic osteoporosis and clarify the mechanism.<h4>Methods</h4>Micro-CT and histology assays were performed to detect the trabecular bone and analyze the bone histomorphology effect of BCA. CCK-8 assay was performed to detect the toxicity of BCA. TRAcP staining, immunofluorescence and hydroxyapatite resorption assay were used to observe osteoclasts2024-01-01T00:00:00Z2024 Jan2026-06-02T06:59:56.303Z2025-04-04T19:22:30.751ZS-EPMC107730523819143810.1186/s12906-023-04332-x