biostudies-literatureDuddeck KAMcNair programUndergraduate Research, Scholarly and Creative Activity (URSCA)-Undergraduate Stipends and ExpensesArm & Hammer Animal and Food ProductionUniversity of Wisconsin-River Falls109https://www.ebi.ac.uk/biostudies/studies/S-EPMC10777967biostudies-literatureUnknown14(1)This experiment was conducted to evaluate the effects of dietary supplementation level of a two-strain <i>Bacillus subtilis</i> probiotic on growth performance, blood parameters, fecal metabolites, and microbiome in nursery pigs. A total of 54 weaned piglets were allotted to three treatments in three replicate pens with six pigs/pen for a 28 d feeding trial. The treatments were as follows: control: no probiotic supplementation; Pro1x: <i>B. subtilis</i> supplementation at 1.875 × 10⁵ CFU/g diet; and Pro10x: <i>B. subtilis</i> supplementation at 1.875 × 10⁶ CFU/g diet. Body weight at d 14 postweaning (<i>p</i> = 0.06) and average daily gain for d 0 to 14 postweaning (<i>p</i> < 0.05) were greater in the Pro1x treatment than in the other treatments. Blood glucose levelsAnimals : an open access journal from MDPIDose-Dependent Effects of Supplementing a Two-Strain <i>Bacillus subtilis</i> Probiotic on Growth Performance, Blood Parameters, Fecal Metabolites, and Microbiome in Nursery Pigs.PMC10777967N/ALi WDuddeck KAWenner SJLarsen AYao DJang YDAdkins HJSmith AHChen CFregulia PPetersen TEHernandez SfalseDose-Dependent Effects of Supplementing a Two-Strain <i>Bacillus subtilis</i> Probiotic on Growth Performance, Blood Parameters, Fecal Metabolites, and Microbiome in Nursery Pigs.This experiment was conducted to evaluate the effects of dietary supplementation level of a two-strain <i>Bacillus subtilis</i> probiotic on growth performance, blood parameters, fecal metabolites, and microbiome in nursery pigs. A total of 54 weaned piglets were allotted to three treatments in three replicate pens with six pigs/pen for a 28 d feeding trial. The treatments were as follows: control: no probiotic supplementation; Pro1x: <i>B. subtilis</i> supplementation at 1.875 × 10⁵ CFU/g diet; and Pro10x: <i>B. subtilis</i> supplementation at 1.875 × 10⁶ CFU/g diet. Body weight at d 14 postweaning (<i>p</i> = 0.06) and average daily gain for d 0 to 14 postweaning (<i>p</i> < 0.05) were greater in the Pro1x treatment than in the other treatments. Blood glucose levels2023-01-01T00:00:00Z2023 Dec2025-08-27T03:08:09.289Z2025-04-04T14:00:02.912ZS-EPMC107779673820084010.3390/ani14010109