biostudies-literatureWang XSpecial Foundation of Guandong Educational CommitteGuangdong Provincial Key Laboratory of Prescription and Syndromed Open Bidding for Selecting the Best Candidates in Guangzhou University 492 of Chinese Medicine21https://www.ebi.ac.uk/biostudies/studies/S-EPMC10779601biostudies-literatureUnknown29(1)Osthole, a natural coumarin found in various medicinal plants, has been previously reported to have neuroprotective effects. However, the specific mechanism by which Osthole alleviates dysmnesia associated with Alzheimer's disease (AD) remains unclear. This study aimed to investigate the neuroprotective properties of Osthole against cognitive impairment in rats induced by D-galactose and elucidate its pharmacological mechanism. The rat model was established by subcutaneously injecting D-galactose at a dose of 150 mg/kg/day for 56 days. The effect of Osthole on cognitive impairment was evaluated by behavior and biochemical analysis. Subsequently, a combination of in silico prediction and experimental validation was performed to verify the network-based predictions, using western blot, NisslMolecules (Basel, Switzerland)Network Proximity Analysis Deciphers the Pharmacological Mechanism of Osthole against D-Galactose Induced Cognitive Disorder in Rats.PMC10779601No. A1-2601-23-414-453Z662022B1212010012(No.2021ZDZX 2001Lai YLiao YCai CFang JFu XFang SWang XLuo XDai ZfalseNetwork Proximity Analysis Deciphers the Pharmacological Mechanism of Osthole against D-Galactose Induced Cognitive Disorder in Rats.Osthole, a natural coumarin found in various medicinal plants, has been previously reported to have neuroprotective effects. However, the specific mechanism by which Osthole alleviates dysmnesia associated with Alzheimer's disease (AD) remains unclear. This study aimed to investigate the neuroprotective properties of Osthole against cognitive impairment in rats induced by D-galactose and elucidate its pharmacological mechanism. The rat model was established by subcutaneously injecting D-galactose at a dose of 150 mg/kg/day for 56 days. The effect of Osthole on cognitive impairment was evaluated by behavior and biochemical analysis. Subsequently, a combination of in silico prediction and experimental validation was performed to verify the network-based predictions, using western blot, Nissl2023-01-01T00:00:00Z2023 Dec2025-04-04T13:58:51.893Z2025-04-04T13:58:51.893ZS-EPMC107796013820260310.3390/molecules29010021