{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhao H"],"funding":["the China Agriculture Program System","National Natural Science Foundation of China","the Program of Science and Technology Construction Xinjiang"],"pagination":["94"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10780974"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["<i>Puccinia striiformis</i> f. sp. <i>tritici</i> is an obligate biotrophic fungus that causes destructive stripe rust disease in wheat. During infection, <i>Pst</i> secretes virulence effectors via a specific infection structure-the haustorium-inside host cells to disturb host immunity and promote fungal colonization and expansion. Hence, the identification and functional analyses of <i>Pst</i> effectors are of great significance in deciphering the <i>Pst</i> pathogenicity mechanism. Here, we identified one candidate <i>Pst</i> effector Pst_9302 that could suppress Bax-triggered cell death in <i>Nicotiana benthamiana</i>. qRT-PCR analyses showed that the transcript levels of <i>Pst_9302</i> were highly increased during the early infection stages of <i>Pst</i>. The transient expression of <i>Pst_9302</i> in wheat via the type-three secretion system (T3SS) significantly inhibited the callose deposition induced by <i>Pseudomonas syringae</i> EtHAn. During wheat-<i>Pst</i> interaction, <i>Pst_9302</i> overexpression suppressed reactive oxygen species (ROS) accumulation and cell death caused by the avirulent <i>Pst</i> race CYR23. The host-induced gene silencing (HIGS) of <i>Pst_9302</i> resulted in decreased <i>Pst</i> pathogenicity with reduced infection area. The results suggest that Pst_9302 plays a virulence role in suppressing plant immunity and promoting <i>Pst</i> pathogenicity. Moreover, wheat voltage-dependent anion channel 1 protein (TaVDAC1) was identified as candidate Pst_9302-interacting proteins by yeast two-hybrid (Y2H) screening. Pull-down assays using the His-Pst_9302 and GST-TaVDAC1 protein verified their interactions. These results suggest that Pst_9302 may modulate wheat TaVDAC1 to regulate plant immunity."],"journal":["Plants (Basel, Switzerland)"],"pubmed_title":["Stripe Rust Effector Pst_9302 Inhibits Wheat Immunity to Promote Susceptibility."],"pmcid":["PMC10780974"],"funding_grant_id":["CARS-03","32161143023；32272511；32302325","2022E02070"],"pubmed_authors":["Nasab HR","Yu L","Zhao C","Huang J","Tang C","Wang X","Zhao H","Zhao X"],"additional_accession":[]},"is_claimable":false,"name":"Stripe Rust Effector Pst_9302 Inhibits Wheat Immunity to Promote Susceptibility.","description":"<i>Puccinia striiformis</i> f. sp. <i>tritici</i> is an obligate biotrophic fungus that causes destructive stripe rust disease in wheat. During infection, <i>Pst</i> secretes virulence effectors via a specific infection structure-the haustorium-inside host cells to disturb host immunity and promote fungal colonization and expansion. Hence, the identification and functional analyses of <i>Pst</i> effectors are of great significance in deciphering the <i>Pst</i> pathogenicity mechanism. Here, we identified one candidate <i>Pst</i> effector Pst_9302 that could suppress Bax-triggered cell death in <i>Nicotiana benthamiana</i>. qRT-PCR analyses showed that the transcript levels of <i>Pst_9302</i> were highly increased during the early infection stages of <i>Pst</i>. The transient expression of <i>Pst_9302</i> in wheat via the type-three secretion system (T3SS) significantly inhibited the callose deposition induced by <i>Pseudomonas syringae</i> EtHAn. During wheat-<i>Pst</i> interaction, <i>Pst_9302</i> overexpression suppressed reactive oxygen species (ROS) accumulation and cell death caused by the avirulent <i>Pst</i> race CYR23. The host-induced gene silencing (HIGS) of <i>Pst_9302</i> resulted in decreased <i>Pst</i> pathogenicity with reduced infection area. The results suggest that Pst_9302 plays a virulence role in suppressing plant immunity and promoting <i>Pst</i> pathogenicity. Moreover, wheat voltage-dependent anion channel 1 protein (TaVDAC1) was identified as candidate Pst_9302-interacting proteins by yeast two-hybrid (Y2H) screening. Pull-down assays using the His-Pst_9302 and GST-TaVDAC1 protein verified their interactions. These results suggest that Pst_9302 may modulate wheat TaVDAC1 to regulate plant immunity.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Dec","modification":"2025-04-04T13:58:23.738Z","creation":"2025-04-04T13:58:23.738Z"},"accession":"S-EPMC10780974","cross_references":{"pubmed":["38202402"],"doi":["10.3390/plants13010094"]}}