biostudies-literatureIorgulescu JBNCI NIH HHSNINDS NIH HHS1297932https://www.ebi.ac.uk/biostudies/studies/S-EPMC10782385biostudies-literatureUnknown14<h4>Background</h4>The GL261 and CT2A syngeneic tumor lines are frequently used as immunocompetent orthotopic mouse models of human glioblastoma (huGBM) but demonstrate distinct differences in their responses to immunotherapy.<h4>Methods</h4>To decipher the cell-intrinsic mechanisms that drive immunotherapy resistance in CT2A-luc and to define the aspects of human cancer biology that these lines can best model, we systematically compared their characteristics using whole exome and transcriptome sequencing, and protein analysis through immunohistochemistry, Western blot, flow cytometry, immunopeptidomics, and phosphopeptidomics.<h4>Results</h4>The transcriptional profiles of GL261-luc2 and CT2A-luc tumors resembled those of some huGBMs, despite neither line sharing the essential genetic or Frontiers in immunologyAntigen presentation deficiency, mesenchymal differentiation, and resistance to immunotherapy in the murine syngeneic CT2A tumor model.PMC10782385K12 CA090354R01 CA208205U01 CA261842P01 CA080124U01 CA224348R01 NS110942P01 CA163205R01 CA259253K22 CA258410R35 CA197743R01 CA269672P01 CA236749Piranlioglu RChiocca EANeagu MPanagioti ESuva MLYates KBEschle BKGokhale PCSharpe AHIorgulescu JBKrishnan SBaker GJReardon DAJain RKFreeman GJWhite FMNeuberg DWu CJDatta MRuthen NSperanza MCSoroko KMAhn RfalseAntigen presentation deficiency, mesenchymal differentiation, and resistance to immunotherapy in the murine syngeneic CT2A tumor model.<h4>Background</h4>The GL261 and CT2A syngeneic tumor lines are frequently used as immunocompetent orthotopic mouse models of human glioblastoma (huGBM) but demonstrate distinct differences in their responses to immunotherapy.<h4>Methods</h4>To decipher the cell-intrinsic mechanisms that drive immunotherapy resistance in CT2A-luc and to define the aspects of human cancer biology that these lines can best model, we systematically compared their characteristics using whole exome and transcriptome sequencing, and protein analysis through immunohistochemistry, Western blot, flow cytometry, immunopeptidomics, and phosphopeptidomics.<h4>Results</h4>The transcriptional profiles of GL261-luc2 and CT2A-luc tumors resembled those of some huGBMs, despite neither line sharing the essential genetic or 2023-01-01T00:00:00Z20232026-06-01T20:19:02.603Z2025-04-04T20:48:12.111ZS-EPMC107823853821332910.3389/fimmu.2023.1297932