biostudies-literatureLiu ZShaanxi Provincial Science and Technology DepartmentNational Natural Science Foundation of China729-741https://www.ebi.ac.uk/biostudies/studies/S-EPMC10787014biostudies-literatureUnknown21(4)Spermatogenesis is a continual process that occurs in the testes, in which diploid spermatogonial stem cells (SSCs) differentiate and generate haploid spermatozoa. This highly efficient and intricate process is orchestrated at multiple levels. N⁶-methyladenosine (m⁶A), an epigenetic modification prevalent in mRNAs, is implicated in the transcriptional regulation during spermatogenesis. However, the dynamics of m⁶A modification in non-rodent mammalian species remains unclear. Here, we systematically investigated the profile and role of m⁶A during spermatogenesis in pigs. By analyzing the transcriptomic distribution of m⁶A in spermatogonia, spermatocytes, and round spermatids, we identified a globally conserved m⁶A pattern betGenomics, proteomics & bioinformaticsTranscriptome-wide Dynamics of m⁶A mRNA Methylation During Porcine Spermatogenesis.PMC10787014817031932020NY-00331572401Zheng YLi FLv YZhang LZeng WZhang PLi XLiu ZHuang TZhang TLu HYu TChen XfalseTranscriptome-wide Dynamics of m⁶A mRNA Methylation During Porcine Spermatogenesis.Spermatogenesis is a continual process that occurs in the testes, in which diploid spermatogonial stem cells (SSCs) differentiate and generate haploid spermatozoa. This highly efficient and intricate process is orchestrated at multiple levels. N⁶-methyladenosine (m⁶A), an epigenetic modification prevalent in mRNAs, is implicated in the transcriptional regulation during spermatogenesis. However, the dynamics of m⁶A modification in non-rodent mammalian species remains unclear. Here, we systematically investigated the profile and role of m⁶A during spermatogenesis in pigs. By analyzing the transcriptomic distribution of m⁶A in spermatogonia, spermatocytes, and round spermatids, we identified a globally conserved m⁶A pattern bet2023-01-01T00:00:00Z2023 Aug2025-04-29T11:32:02.122Z2025-04-06T19:54:11.995ZS-EPMC107870143454372310.1016/j.gpb.2021.08.006