biostudies-literatureLe Franc LCNRSEcosphère Continentale et Côtière ’HERITAGe’Guillaume Rivière and the Council of the Normandy Region742-755https://www.ebi.ac.uk/biostudies/studies/S-EPMC10787124biostudies-literatureUnknown21(4)The N⁶-methylation of RNA adenosines (N⁶-methyladenosine, m⁶A) is an important regulator of gene expression with critical implications in vertebrate and insect development. However, the developmental significance of epitranscriptomes in lophotrochozoan organisms remains unknown. Using methylated RNA immunoprecipitation sequencing (MeRIP-seq), we generated transcriptome-wide m⁶A-RNA methylomes covering the entire development of the oyster from oocytes to juveniles. Oyster RNA classes display specific m⁶A signatures, with messenger RNAs (mRNAs) and long non-coding RNAs (lncRNAs) exhibiting distinct profiles and being highly methylated compared to transposable element (TE) transcripts. Epitranscriptomes are dynamic and correspond to the cGenomics, proteomics & bioinformaticsm⁶A Profile Dynamics Indicates Regulation of Oyster Development by m⁶A-RNA Epitranscriptomes.PMC107871242019-200919362L18E01643-18P02383Riviere GLe Franc LPetton BFavrel Pfalsem⁶A Profile Dynamics Indicates Regulation of Oyster Development by m⁶A-RNA Epitranscriptomes.The N⁶-methylation of RNA adenosines (N⁶-methyladenosine, m⁶A) is an important regulator of gene expression with critical implications in vertebrate and insect development. However, the developmental significance of epitranscriptomes in lophotrochozoan organisms remains unknown. Using methylated RNA immunoprecipitation sequencing (MeRIP-seq), we generated transcriptome-wide m⁶A-RNA methylomes covering the entire development of the oyster from oocytes to juveniles. Oyster RNA classes display specific m⁶A signatures, with messenger RNAs (mRNAs) and long non-coding RNAs (lncRNAs) exhibiting distinct profiles and being highly methylated compared to transposable element (TE) transcripts. Epitranscriptomes are dynamic and correspond to the c2023-01-01T00:00:00Z2023 Aug2026-07-03T03:21:38.661Z2024-11-21T03:37:43.791ZS-EPMC107871243649612910.1016/j.gpb.2022.12.002