{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Roodsant TJ"],"funding":["EC | Horizon 2020 Framework Programme (H2020)","EC | Horizon 2020 Framework Programme"],"pagination":["e0225923"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10790761"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["<h4>Importance</h4>Phase variation allows a single strain to produce phenotypic diverse subpopulations. Phase-variable restriction modification (RM) systems are systems that allow for such phase variation via epigenetic regulation of gene expression levels. The phase-variable RM system SsuCC20p was found in multiple streptococcal species and was acquired by an emerging zoonotic lineage of <i>Streptococcus suis</i>. We show that the phase variability of SsuCC20p is dependent on a recombinase encoded within the SsuCC20p locus. We characterized the genome methylation profiles of the different phases of SsuCC20p and demonstrated the consequential impact on the transcriptome and virulence in a zebrafish infection model. Acquiring mobile genetic elements containing epigenetic regulatory systems, like phase-variable RM systems, enables bacterial pathogens to produce diverse phenotypic subpopulations that are better adapted to specific (host) environments encountered during infection."],"journal":["mBio"],"pubmed_title":["The streptococcal phase-variable type I restriction modification system SsuCC20p dictates the methylome of <i>Streptococcus suis</i> impacting the transcriptome and virulence in a zebrafish larvae infection model."],"pmcid":["PMC10790761"],"funding_grant_id":["727966"],"pubmed_authors":["Roodsant TJ","Schultsz C","Baltussen TJH","van der Ark KCH","van der Putten B","Brizuela J","Coolen JPM","Pannekoek Y","Schipper K"],"additional_accession":[]},"is_claimable":false,"name":"The streptococcal phase-variable type I restriction modification system SsuCC20p dictates the methylome of <i>Streptococcus suis</i> impacting the transcriptome and virulence in a zebrafish larvae infection model.","description":"<h4>Importance</h4>Phase variation allows a single strain to produce phenotypic diverse subpopulations. Phase-variable restriction modification (RM) systems are systems that allow for such phase variation via epigenetic regulation of gene expression levels. The phase-variable RM system SsuCC20p was found in multiple streptococcal species and was acquired by an emerging zoonotic lineage of <i>Streptococcus suis</i>. We show that the phase variability of SsuCC20p is dependent on a recombinase encoded within the SsuCC20p locus. We characterized the genome methylation profiles of the different phases of SsuCC20p and demonstrated the consequential impact on the transcriptome and virulence in a zebrafish infection model. Acquiring mobile genetic elements containing epigenetic regulatory systems, like phase-variable RM systems, enables bacterial pathogens to produce diverse phenotypic subpopulations that are better adapted to specific (host) environments encountered during infection.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jan","modification":"2026-06-01T11:26:39.737Z","creation":"2025-06-01T01:57:07.786Z"},"accession":"S-EPMC10790761","cross_references":{"pubmed":["38063379"],"doi":["10.1128/mbio.02259-23"]}}