biostudies-literatureUnknown6(2)Tricase AHerein, we describe a novel method for producing cadmium-selenide nanoparticles (CdSe NPs) with controlled size using apoferritin as a bionanoreactor triggered by local pH change at the electrode/solution interface. Apoferritin is known for its reversible self-assembly at alkaline pH. The pH change is induced electrochemically by reducing O₂ through the application of sufficiently negative voltages and bioelectrochemically through O₂ reduction catalyzed by laccase, co-immobilized with apoferritin on the electrode surface. Specifically, a Ti electrode is modified with (3-aminopropyl)triethoxysilane, followed by glutaraldehyde cross-linking (1.5% v/v in H₂O) of apoferritin (as the bionanoreactor) and laccase (as the local pH change triggering system). This prNanoscale advances516-523https://www.ebi.ac.uk/biostudies/studies/S-EPMC10790968biostudies-literatureBioelectrochemically triggered apoferritin-based bionanoreactors: synthesis of CdSe nanoparticles and monitoring with leaky waveguides.PMC10790968Gupta RBollella PMarchiano VTorsi LTricase AAlhenaki BfalseBioelectrochemically triggered apoferritin-based bionanoreactors: synthesis of CdSe nanoparticles and monitoring with leaky waveguides.Herein, we describe a novel method for producing cadmium-selenide nanoparticles (CdSe NPs) with controlled size using apoferritin as a bionanoreactor triggered by local pH change at the electrode/solution interface. Apoferritin is known for its reversible self-assembly at alkaline pH. The pH change is induced electrochemically by reducing O₂ through the application of sufficiently negative voltages and bioelectrochemically through O₂ reduction catalyzed by laccase, co-immobilized with apoferritin on the electrode surface. Specifically, a Ti electrode is modified with (3-aminopropyl)triethoxysilane, followed by glutaraldehyde cross-linking (1.5% v/v in H₂O) of apoferritin (as the bionanoreactor) and laccase (as the local pH change triggering system). This pr2024-01-01T00:00:00Z2024 Jan2025-04-03T22:42:54.287Z2024-10-16T09:04:11.601ZS-EPMC107909683823509410.1039/d3na01046e