{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14"],"submitter":["Santoni M"],"pubmed_abstract":["The COVID-19 pandemic has underscored the need for rapid and cost-effective diagnostic tools. Serological tests, particularly those measuring antibodies targeting the receptor-binding domain (RBD) of the virus, play a pivotal role in tracking infection dynamics and vaccine effectiveness. In this study, we aimed to develop a simple enzyme-linked immunosorbent assay (ELISA) for measuring RBD-specific antibodies, comparing two plant-based platforms for diagnostic reagent production. We chose to retain RBD in the endoplasmic reticulum (ER) to prevent potential immunoreactivity issues associated with plant-specific glycans. We produced ER-retained RBD in two plant systems: a stable transformation of BY-2 plant cell culture (BY2-RBD) and a transient transformation in <i>Nicotiana benthamiana</i>"],"journal":["Frontiers in plant science"],"pagination":["1325162"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10794598"],"repository":["biostudies-literature"],"pubmed_title":["Performance of plant-produced RBDs as SARS-CoV-2 diagnostic reagents: a tale of two plant platforms."],"pmcid":["PMC10794598"],"pubmed_authors":["Pivotto D","Zanichelli E","Bardor M","Mazzariol A","Avesani L","Ritala A","Gutierrez-Valdes N","Compri M","Santoni M","Rosa A","Sobrino-Mengual G","Lerouge P","Cecchetto R","Balieu J"],"additional_accession":[]},"is_claimable":false,"name":"Performance of plant-produced RBDs as SARS-CoV-2 diagnostic reagents: a tale of two plant platforms.","description":"The COVID-19 pandemic has underscored the need for rapid and cost-effective diagnostic tools. Serological tests, particularly those measuring antibodies targeting the receptor-binding domain (RBD) of the virus, play a pivotal role in tracking infection dynamics and vaccine effectiveness. In this study, we aimed to develop a simple enzyme-linked immunosorbent assay (ELISA) for measuring RBD-specific antibodies, comparing two plant-based platforms for diagnostic reagent production. We chose to retain RBD in the endoplasmic reticulum (ER) to prevent potential immunoreactivity issues associated with plant-specific glycans. We produced ER-retained RBD in two plant systems: a stable transformation of BY-2 plant cell culture (BY2-RBD) and a transient transformation in <i>Nicotiana benthamiana</i>","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2026-04-15T03:21:51.259Z","creation":"2024-10-16T00:13:48.592Z"},"accession":"S-EPMC10794598","cross_references":{"pubmed":["38239207"],"doi":["10.3389/fpls.2023.1325162"]}}