{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Vargas DA"],"funding":["NIAID NIH HHS","FIC NIH HHS","Wellcome Trust"],"pagination":["1242727"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10795579"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2"],"pubmed_abstract":["Host cell functions that participate in the pharmacokinetics and pharmacodynamics (PK/PD) of drugs against intracellular pathogen infections are critical for drug efficacy. In this study, we investigated whether macrophage mechanisms of xenobiotic detoxification contribute to the elimination of intracellular <i>Leishmania</i> upon exposure to pentavalent antimonials (Sb<sup>V</sup>). Primary macrophages from patients with cutaneous leishmaniasis (CL) (n=6) were exposed <i>ex vivo</i> to <i>L. V. panamensis</i> infection and Sb<sup>V</sup>, and transcriptomes were generated. Seven metallothionein (MT) genes, potent scavengers of heavy metals and central elements of the mammalian cell machinery for xenobiotic detoxification, were within the top 20 up-regulated genes. To functionally validate"],"journal":["Frontiers in parasitology"],"pubmed_title":["Macrophage metallothioneins participate in the antileishmanial activity of antimonials."],"pmcid":["PMC10795579"],"funding_grant_id":["107595/Z/15/Z","D43 TW006589","R01 AI104823","U19 AI129910"],"pubmed_authors":["Zilberstein D","Koren RN","Gomez MA","Gregory DJ","El-Sayed NM","Belew AT","Vargas DA"],"additional_accession":[]},"is_claimable":false,"name":"Macrophage metallothioneins participate in the antileishmanial activity of antimonials.","description":"Host cell functions that participate in the pharmacokinetics and pharmacodynamics (PK/PD) of drugs against intracellular pathogen infections are critical for drug efficacy. In this study, we investigated whether macrophage mechanisms of xenobiotic detoxification contribute to the elimination of intracellular <i>Leishmania</i> upon exposure to pentavalent antimonials (Sb<sup>V</sup>). Primary macrophages from patients with cutaneous leishmaniasis (CL) (n=6) were exposed <i>ex vivo</i> to <i>L. V. panamensis</i> infection and Sb<sup>V</sup>, and transcriptomes were generated. Seven metallothionein (MT) genes, potent scavengers of heavy metals and central elements of the mammalian cell machinery for xenobiotic detoxification, were within the top 20 up-regulated genes. To functionally validate","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2026-06-02T06:11:20.163Z","creation":"2024-10-16T00:11:48.606Z"},"accession":"S-EPMC10795579","cross_references":{"pubmed":["38239429"],"doi":["10.3389/fpara.2023.1242727"]}}