biostudies-literatureKoltai GDeutsche ForschungsgemeinschaftHeidelberg Graduate School of Fundamental PhysicsUniversität Innsbruck1553https://www.ebi.ac.uk/biostudies/studies/S-EPMC10796908biostudies-literatureUnknown14(1)Carbonate geothermometry is a fundamental tool for quantitative assessment of the geothermal and geochemical evolution of diagenetic and hydrothermal systems, but it remains difficult to obtain accurate and precise formation temperatures of low-temperature calcite samples (below ~ 40 to 60 °C). Here, we apply three geothermometry methods (∆₄₇-thermometry, nucleation-assisted fluid inclusion microthermometry-hereafter NA-FIM-and oxygen isotope thermometry) to slow-growing subaqueous calcite spar samples to cross-validate these methods down to 10 °C. Temperatures derived by NA-FIM and Δ₄₇-thermometry agree within the 95% confidence interval, except for one sample. Regression analyses suggest that the real uncertainty of ∆₄₇-thermometry exceeds the 1 SE analytScientific reportsGeothermometry of calcite spar at 10-50 °C.PMC10796908GSC-129W17150935/1270-1 FUGGLeel-Ossy SDublyansky YKluge THoniat CSpotl CKoltai GRinyu LAudra PKruger YfalseGeothermometry of calcite spar at 10-50 °C.Carbonate geothermometry is a fundamental tool for quantitative assessment of the geothermal and geochemical evolution of diagenetic and hydrothermal systems, but it remains difficult to obtain accurate and precise formation temperatures of low-temperature calcite samples (below ~ 40 to 60 °C). Here, we apply three geothermometry methods (∆₄₇-thermometry, nucleation-assisted fluid inclusion microthermometry-hereafter NA-FIM-and oxygen isotope thermometry) to slow-growing subaqueous calcite spar samples to cross-validate these methods down to 10 °C. Temperatures derived by NA-FIM and Δ₄₇-thermometry agree within the 95% confidence interval, except for one sample. Regression analyses suggest that the real uncertainty of ∆₄₇-thermometry exceeds the 1 SE analyt2024-01-01T00:00:00Z2024 Jan2025-05-18T11:10:18.087Z2024-10-17T21:54:11.229ZS-EPMC107969083823841210.1038/s41598-024-51937-4