{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wei Y"],"funding":["China Agricultural Research System","Local grants","Sci-Tech Innovation 2030 Key Program","National Natural Science Foundation of China"],"pagination":["e2304113"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10797475"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(3)"],"pubmed_abstract":["Mitochondrial base editing with DddA-derived cytosine base editor (DdCBE) is limited in the accessible target sequences and modest activity. Here, the optimized DdCBE tools is presented with improved editing activity and expanded C-to-T targeting scope by fusing DddA11 variant with different cytosine deaminases with single-strand DNA activity. Compared to previous DdCBE based on DddA11 variant alone, fusion of the activation-induced cytidine deaminase (AID) from Xenopus laevis not only permits cytosine editing of 5'-GC-3' sequence, but also elevates editing efficiency at 5'-TC-3', 5'-CC-3', and 5'-GC-3' targets by up to 25-, 10-, and 6-fold, respectively. Furthermore, the A-to-G editing efficiency is significantly improved by fusing the evolved DddA6 variant with TALE-linked deoxyadenosine"],"journal":["Advanced science (Weinheim, Baden-Wurttemberg, Germany)"],"pubmed_title":["Enhanced C-To-T and A-To-G Base Editing in Mitochondrial DNA with Engineered DdCBE and TALED."],"pmcid":["PMC10797475"],"funding_grant_id":["2022ZD0401401","Z1013023006","2023ZD0405104","32272848","2022GD-TSLD-46","CARS‐39‐03","CARS-39-03","32301251"],"pubmed_authors":["Yang H","Xu K","Xu C","Zhang M","Zhou Y","Wei Y","Li W","Huang S","Gao P","Li S","Jin M","Ren N","Chen Y","Wang X","Yao F"],"additional_accession":[]},"is_claimable":false,"name":"Enhanced C-To-T and A-To-G Base Editing in Mitochondrial DNA with Engineered DdCBE and TALED.","description":"Mitochondrial base editing with DddA-derived cytosine base editor (DdCBE) is limited in the accessible target sequences and modest activity. Here, the optimized DdCBE tools is presented with improved editing activity and expanded C-to-T targeting scope by fusing DddA11 variant with different cytosine deaminases with single-strand DNA activity. Compared to previous DdCBE based on DddA11 variant alone, fusion of the activation-induced cytidine deaminase (AID) from Xenopus laevis not only permits cytosine editing of 5'-GC-3' sequence, but also elevates editing efficiency at 5'-TC-3', 5'-CC-3', and 5'-GC-3' targets by up to 25-, 10-, and 6-fold, respectively. Furthermore, the A-to-G editing efficiency is significantly improved by fusing the evolved DddA6 variant with TALE-linked deoxyadenosine","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jan","modification":"2026-06-02T02:23:33.141Z","creation":"2025-04-04T19:22:08.874Z"},"accession":"S-EPMC10797475","cross_references":{"pubmed":["37984866"],"doi":["10.1002/advs.202304113"]}}