{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rospo G"],"funding":["AIRC under IG 2018","International Accelerator Award, ACRCelerate","Fondazione AIRC under 5 per Mille 2018","Cancer Research UK","European Research Council","AIRC MFAG 2020","IMI PERSIST-SEQ","AIRC 5x1000"],"pagination":["15"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10797964"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["<h4>Background</h4>Immunotherapy based on checkpoint inhibitors is highly effective in mismatch repair deficient (MMRd) colorectal cancer (CRC). These tumors carry a high number of mutations, which are predicted to translate into a wide array of neoepitopes; however, a systematic classification of the neoantigen repertoire in MMRd CRC is lacking. Mass spectrometry peptidomics has demonstrated the existence of MHC class I associated peptides (MAPs) originating from non-coding DNA regions. Based on these premises we investigated DNA genomic regions responsible for generating MMRd-induced peptides.<h4>Methods</h4>We exploited mouse CRC models in which the MMR gene Mlh1 was genetically inactivated. Isogenic cell lines CT26 Mlh1<sup>+/+</sup> and Mlh1<sup>-/-</sup> were inoculated in immunocomp"],"journal":["Genome medicine"],"pubmed_title":["Non-canonical antigens are the largest fraction of peptides presented by MHC class I in mismatch repair deficient murine colorectal cancer."],"pmcid":["PMC10797964"],"funding_grant_id":["21763","22737","A28223","contract n. 101007937","22795","GEACC18004TAB","ID. 21923 project","A26825","Grant-ID 24604","Cancer Research UK (A26825 and A28223), FC AECC (GEACC18004TAB), and AIRC (22795)","ID. 21091 program","101020342","grant agreement no.101020342"],"pubmed_authors":["Bartolini A","Germano G","Bachi A","Matafora V","Mondino A","Bardelli A","Lamba S","Chila R","Basso V","Di Nicolantonio F","Rospo G"],"additional_accession":[]},"is_claimable":false,"name":"Non-canonical antigens are the largest fraction of peptides presented by MHC class I in mismatch repair deficient murine colorectal cancer.","description":"<h4>Background</h4>Immunotherapy based on checkpoint inhibitors is highly effective in mismatch repair deficient (MMRd) colorectal cancer (CRC). These tumors carry a high number of mutations, which are predicted to translate into a wide array of neoepitopes; however, a systematic classification of the neoantigen repertoire in MMRd CRC is lacking. Mass spectrometry peptidomics has demonstrated the existence of MHC class I associated peptides (MAPs) originating from non-coding DNA regions. Based on these premises we investigated DNA genomic regions responsible for generating MMRd-induced peptides.<h4>Methods</h4>We exploited mouse CRC models in which the MMR gene Mlh1 was genetically inactivated. Isogenic cell lines CT26 Mlh1<sup>+/+</sup> and Mlh1<sup>-/-</sup> were inoculated in immunocomp","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jan","modification":"2026-07-15T04:47:20.356Z","creation":"2025-04-04T19:22:14.289Z"},"accession":"S-EPMC10797964","cross_references":{"pubmed":["38243308"],"doi":["10.1186/s13073-023-01275-3"]}}