biostudies-literatureZhang DProjects of Science and Technology Department of Sichuan ProvinceThe National 973 ProjectScience and Technology Department of Chengdu CityNational Natural Science Foundation of ChinaJapan Society for the Promotion of Sciencee13397https://www.ebi.ac.uk/biostudies/studies/S-EPMC10799210biostudies-literatureUnknown25(1)Rhizoctonia solani AG-1 IA causes a necrotrophic rice disease and is a serious threat to rice production. To date, only a few effectors have been characterized in AG-1 IA. We previously identified RsIA_CtaG/Cox11 and showed that infiltration of the recombinant protein into rice leaves caused disease-like symptoms. In the present study, we further characterized the functionality of RsIA_CtaG/Cox11. RsIA_CtaG/Cox11 is an alternative transcript of cytochrome c oxidase copper chaperone Cox11 that starts from the second AUG codon, but contains a functional secretion signal peptide. RNA interference with RsIA_CtaG/Cox11 reduced the pathogenicity of AG-1 IA towards rice and Nicotiana benthamiana without affecting its fitness or mycelial morphology. Transient expression of the RsIA_CtaG/Cox11-GFP Molecular plant pathologyMitochondrial-targeting effector RsIA_CtaG/Cox11 in Rhizoctonia solani AG-1 IA has two functions: plant immunity suppression and cell death induction mediated by a rice cytochrome c oxidase subunit.PMC1079921021H021972021-YF05-02326-SN2019YFN00102021-GH02-00085-HZ2020-YFH016118H02206314001302014CB160304Yamamoto NYi XZhang DLin ROkada KNoutoshi YZheng TLiu YZheng HLin HXiang XZheng AWang ZfalseMitochondrial-targeting effector RsIA_CtaG/Cox11 in Rhizoctonia solani AG-1 IA has two functions: plant immunity suppression and cell death induction mediated by a rice cytochrome c oxidase subunit.Rhizoctonia solani AG-1 IA causes a necrotrophic rice disease and is a serious threat to rice production. To date, only a few effectors have been characterized in AG-1 IA. We previously identified RsIA_CtaG/Cox11 and showed that infiltration of the recombinant protein into rice leaves caused disease-like symptoms. In the present study, we further characterized the functionality of RsIA_CtaG/Cox11. RsIA_CtaG/Cox11 is an alternative transcript of cytochrome c oxidase copper chaperone Cox11 that starts from the second AUG codon, but contains a functional secretion signal peptide. RNA interference with RsIA_CtaG/Cox11 reduced the pathogenicity of AG-1 IA towards rice and Nicotiana benthamiana without affecting its fitness or mycelial morphology. Transient expression of the RsIA_CtaG/Cox11-GFP 2024-01-01T00:00:00Z2024 Jan2025-05-18T11:10:55.674Z2024-10-17T21:57:38.12ZS-EPMC107992103790258910.1111/mpp.13397