biostudies-literatureUnknown11(4)Rajora MANatural Sciences and Engineering Research Council of CanadaPrincess Margaret Cancer FoundationTerry Fox Research InstitutePeterborough K. M. Hunter Charitable FoundationMcLaughlin Centre, University of TorontoProstate Cancer CanadaCanada Foundation for InnovationCanadian Institutes of Health ResearchInstitute of Cancer ResearchCanada Research ChairsCIHRMicrobubble-enabled focused ultrasound (MB-FUS) has revolutionized nano and molecular drug delivery capabilities. Yet, the absence of longitudinal, systematic, quantitative studies of microbubble shell pharmacokinetics hinders progress within the MB-FUS field. Microbubble radiolabeling challenges contribute to this void. This barrier is overcome by developing a one-pot, purification-free copper chelation protocol able to stably radiolabel diverse porphyrin-lipid-containing Definity® analogues (pDefs) with >95% efficiency while maintaining microbubble physicochemical properties. Five tri-modal (ultrasound-, positron emission tomography (PET)-, and fluorescent-active) [⁶⁴ Cu]Cu-pDefs are created with varying lipid acyl chain length and charge, representing the most prevalently stuAdvanced science (Weinheim, Baden-Wurttemberg, Germany)e2304453https://www.ebi.ac.uk/biostudies/studies/S-EPMC10811482biostudies-literatureQuantitative Pharmacokinetics Reveal Impact of Lipid Composition on Microbubble and Nanoprogeny Shell Fate.PMC10811482Dhaliwal AGoertz DPellow CChen JZheng MChoi VZheng GRajora MAOverchuk MLou JWHfalseQuantitative Pharmacokinetics Reveal Impact of Lipid Composition on Microbubble and Nanoprogeny Shell Fate.Microbubble-enabled focused ultrasound (MB-FUS) has revolutionized nano and molecular drug delivery capabilities. Yet, the absence of longitudinal, systematic, quantitative studies of microbubble shell pharmacokinetics hinders progress within the MB-FUS field. Microbubble radiolabeling challenges contribute to this void. This barrier is overcome by developing a one-pot, purification-free copper chelation protocol able to stably radiolabel diverse porphyrin-lipid-containing Definity® analogues (pDefs) with >95% efficiency while maintaining microbubble physicochemical properties. Five tri-modal (ultrasound-, positron emission tomography (PET)-, and fluorescent-active) [⁶⁴ Cu]Cu-pDefs are created with varying lipid acyl chain length and charge, representing the most prevalently stu2024-01-01T00:00:00Z2024 Jan2025-04-04T09:17:54.134Z2025-04-04T09:17:54.134ZS-EPMC108114823803212910.1002/advs.202304453