biostudies-literatureLiu MJilin Provincial Department of Science and Technology Program285https://www.ebi.ac.uk/biostudies/studies/S-EPMC10812751biostudies-literatureUnknown14(2)The ubiquitous Gram-negative bacterial pathogen <i>Aeromonas veronii</i> (<i>A. veronii</i>) can easily cause inflammatory reactions in aquatic organisms, resulting in high mortality and huge economic losses. MicroRNAs (miRNAs) participate in immune regulation and have certain conserved properties. MiRNAs are involved in the immune responses of a variety of teleost fish infected with bacteria, whereas there is no related report in silver carp (<i>Hypophthalmichthys molitrix</i>). Therefore, we identified the expression profiles of miRNA in silver carp stimulated by <i>A. veronii</i> and LPS. Among them, the quantity of differentially expressed miRNAs (DEmiRNAs) obtained in the silver carp challenge group was 73 (<i>A. veronii</i>) and 90 (LPS). The GO enrichment and analysis of KEGG pathwaAnimals : an open access journal from MDPIIdentification and Characterization of Immune-Associated MicroRNAs in Silver Carp (<i>Hypophthalmichthys molitrix</i>) Responding to <i>Aeromonas veronii</i> and LPS Stimulation.PMC1081275120210202049NCYang ZShan XLiu MGao KZhang XGao YTang HfalseIdentification and Characterization of Immune-Associated MicroRNAs in Silver Carp (<i>Hypophthalmichthys molitrix</i>) Responding to <i>Aeromonas veronii</i> and LPS Stimulation.The ubiquitous Gram-negative bacterial pathogen <i>Aeromonas veronii</i> (<i>A. veronii</i>) can easily cause inflammatory reactions in aquatic organisms, resulting in high mortality and huge economic losses. MicroRNAs (miRNAs) participate in immune regulation and have certain conserved properties. MiRNAs are involved in the immune responses of a variety of teleost fish infected with bacteria, whereas there is no related report in silver carp (<i>Hypophthalmichthys molitrix</i>). Therefore, we identified the expression profiles of miRNA in silver carp stimulated by <i>A. veronii</i> and LPS. Among them, the quantity of differentially expressed miRNAs (DEmiRNAs) obtained in the silver carp challenge group was 73 (<i>A. veronii</i>) and 90 (LPS). The GO enrichment and analysis of KEGG pathwa2024-01-01T00:00:00Z2024 Jan2026-07-03T03:22:12.844Z2025-04-21T15:59:02.089ZS-EPMC108127513825445410.3390/ani14020285