biostudies-literatureMarzucco Athe European Union-NextGenerationEU through the Italian Ministry of University and Research under PNRR-Mission 4 Component 2, Investment 3.3 "Partnerships extended to universities, re-search centresMonica Cricca153https://www.ebi.ac.uk/biostudies/studies/S-EPMC10820201biostudies-literatureUnknown12(1)<i>Candida</i> spp. are an important opportunistic pathogen that can represent a possible cause of severe infections, especially in immunocompromised individuals. The clinical impact of <i>Candida</i> spp. depends, in part, on the ability to form biofilms, communities of nestled cells into the extracellular matrix. In this study, we compared the biofilm formation ability of 83 strains of <i>Candida</i> spp. isolated from blood cultures and other materials, such as respiratory samples, urine, and exudate, and their sensitivity to fluconazole (FLZ). Strains were divided into tertiles to establish cut-offs to classify isolates as low, moderate, or high biofilm producers (<0.26, 0.266-0.839, >0.839) and biofilms with low, moderate, or high metabolic activity (<0.053, 0.053-0.183, >0.183). A noMicroorganismsEvaluation of Biofilm Production and Antifungal Susceptibility to Fluconazole in Clinical Isolates of <i>Candida</i> spp. in Both Planktonic and Biofilm Form.PMC10820201Ricerca Fondamentale Orientata (RFO) 2021 and 2022352/2021 - CUP J33C22001330009Tamburini MVCongestri FBrandolini MTaddei FColosimo CMontanari MSDirani GSambri VZannoli SSchiavone PGatti GFantini MMorotti MManera MCricca MArfilli VGrumiro LDe Pascali AMMarzucco ADenicolo AfalseEvaluation of Biofilm Production and Antifungal Susceptibility to Fluconazole in Clinical Isolates of <i>Candida</i> spp. in Both Planktonic and Biofilm Form.<i>Candida</i> spp. are an important opportunistic pathogen that can represent a possible cause of severe infections, especially in immunocompromised individuals. The clinical impact of <i>Candida</i> spp. depends, in part, on the ability to form biofilms, communities of nestled cells into the extracellular matrix. In this study, we compared the biofilm formation ability of 83 strains of <i>Candida</i> spp. isolated from blood cultures and other materials, such as respiratory samples, urine, and exudate, and their sensitivity to fluconazole (FLZ). Strains were divided into tertiles to establish cut-offs to classify isolates as low, moderate, or high biofilm producers (<0.26, 0.266-0.839, >0.839) and biofilms with low, moderate, or high metabolic activity (<0.053, 0.053-0.183, >0.183). A no2024-01-01T00:00:00Z2024 Jan2025-04-04T09:16:59.013Z2025-04-04T09:16:59.013ZS-EPMC108202013825798010.3390/microorganisms12010153