biostudies-literatureLu TCNIA NIH HHSNIDCD NIH HHSNINDS NIH HHSNCI NIH HHSNIGMS NIH HHSeadg0934https://www.ebi.ac.uk/biostudies/studies/S-EPMC10829769biostudies-literatureUnknown380(6650)Aging is characterized by a decline in tissue function, but the underlying changes at cellular resolution across the organism remain unclear. Here, we present the Aging Fly Cell Atlas, a single-nucleus transcriptomic map of the whole aging <i>Drosophila</i>. We characterized 163 distinct cell types and performed an in-depth analysis of changes in tissue cell composition, gene expression, and cell identities. We further developed aging clock models to predict fly age and show that ribosomal gene expression is a conserved predictive factor for age. Combining all aging features, we find distinctive cell type-specific aging patterns. This atlas provides a valuable resource for studying fundamental principles of aging in complex organisms.Science (New York, N.Y.)Aging Fly Cell Atlas identifies exhaustive aging features at cellular resolution.PMC10829769R01 NS083833P30 CA008748T32 GM136560R01 DC005982R00 AG062746Brbic MLee SDing SHChen YCPisco AOCai XTLiang CYWelsch DPark YJQi YKatheder NSLi HLu TCJasper HD'Souza SJackson TChen JLeskovec JAuld NLuo LQuake SRJones RCLai ECKolluru SSBellen HJfalseAging Fly Cell Atlas identifies exhaustive aging features at cellular resolution.Aging is characterized by a decline in tissue function, but the underlying changes at cellular resolution across the organism remain unclear. Here, we present the Aging Fly Cell Atlas, a single-nucleus transcriptomic map of the whole aging <i>Drosophila</i>. We characterized 163 distinct cell types and performed an in-depth analysis of changes in tissue cell composition, gene expression, and cell identities. We further developed aging clock models to predict fly age and show that ribosomal gene expression is a conserved predictive factor for age. Combining all aging features, we find distinctive cell type-specific aging patterns. This atlas provides a valuable resource for studying fundamental principles of aging in complex organisms.2023-01-01T00:00:00Z2023 Jun2025-04-26T10:29:57.161Z2025-04-06T13:23:40.571ZS-EPMC108297693731921210.1126/science.adg0934