biostudies-literatureUnknown8Lin WLThe crisp grass carp (CGC; <i>Ctenopharyngodon idellus</i> C. et V.), known for its unique texture and flavour, is a culinary delicacy whose quality is significantly influenced by thermal processing. This study employed 4D label-free proteomics and data mining techniques to investigate the proteomic changes in CGC muscle tissue induced by various heating temperatures. CGC samples were subjected to a series of heat treatments at increasing temperatures from 20 °C to 90 °C. Proteins were extracted, digested, and analysed using high-resolution mass spectrometry. The proteomic data were then subjected to extensive bioinformatics analysis, including GO and KEGG pathway enrichment. We identified a total of 1085 proteins, 516 of which were shared across all the temperature treatments, indicating Current research in food science100681https://www.ebi.ac.uk/biostudies/studies/S-EPMC10832373biostudies-literatureIntegrated 4D label-free proteomics and data mining to elucidate the effects of thermal processing on crisp grass carp protein profiles.PMC10832373Li LHChen SJWang YQLiu HXHuang HLiu YQWei YWu YYLin WLZhao YQfalseIntegrated 4D label-free proteomics and data mining to elucidate the effects of thermal processing on crisp grass carp protein profiles.The crisp grass carp (CGC; <i>Ctenopharyngodon idellus</i> C. et V.), known for its unique texture and flavour, is a culinary delicacy whose quality is significantly influenced by thermal processing. This study employed 4D label-free proteomics and data mining techniques to investigate the proteomic changes in CGC muscle tissue induced by various heating temperatures. CGC samples were subjected to a series of heat treatments at increasing temperatures from 20 °C to 90 °C. Proteins were extracted, digested, and analysed using high-resolution mass spectrometry. The proteomic data were then subjected to extensive bioinformatics analysis, including GO and KEGG pathway enrichment. We identified a total of 1085 proteins, 516 of which were shared across all the temperature treatments, indicating 2024-01-01T00:00:00Z20242025-06-01T12:02:41.947Z2025-06-01T12:02:41.947ZS-EPMC108323733830400010.1016/j.crfs.2024.100681