{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li W"],"funding":["NEI NIH HHS"],"pagination":["17-41"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10834862"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["30"],"pubmed_abstract":["<h4>Purpose</h4>The conserved miR-183/96/182 cluster (miR-183C) regulates both corneal sensory innervation and corneal resident immune cells (CRICs). This study is to uncover its role in CRICs and in shaping the corneal cellular landscape at a single-cell (sc) level.<h4>Methods</h4>Corneas of naïve, young adult [2 and 6 months old (mo)], female miR-183C knockout (KO) mice and wild-type (WT) littermates were harvested and dissociated into single cells. Dead cells were removed using a Dead Cell Removal kit. CD45<sup>+</sup> CRICs were enriched by Magnetic Activated Cell Sorting (MACS). scRNA libraries were constructed and sequenced followed by comprehensive bioinformatic analyses.<h4>Results</h4>The composition of major cell types of the cornea stays relatively stable in WT mice from 2 to 6 "],"journal":["The ocular surface"],"pubmed_title":["The miR-183/96/182 cluster is a checkpoint for resident immune cells and shapes the cellular landscape of the cornea."],"pmcid":["PMC10834862"],"funding_grant_id":["R01 EY026059","P30 EY004068","R01 EY016058"],"pubmed_authors":["Li W","Gurdziel K","Pitchaikannu A","Hazlett LD","Xu S","Gupta N"],"additional_accession":[]},"is_claimable":false,"name":"The miR-183/96/182 cluster is a checkpoint for resident immune cells and shapes the cellular landscape of the cornea.","description":"<h4>Purpose</h4>The conserved miR-183/96/182 cluster (miR-183C) regulates both corneal sensory innervation and corneal resident immune cells (CRICs). This study is to uncover its role in CRICs and in shaping the corneal cellular landscape at a single-cell (sc) level.<h4>Methods</h4>Corneas of naïve, young adult [2 and 6 months old (mo)], female miR-183C knockout (KO) mice and wild-type (WT) littermates were harvested and dissociated into single cells. Dead cells were removed using a Dead Cell Removal kit. CD45<sup>+</sup> CRICs were enriched by Magnetic Activated Cell Sorting (MACS). scRNA libraries were constructed and sequenced followed by comprehensive bioinformatic analyses.<h4>Results</h4>The composition of major cell types of the cornea stays relatively stable in WT mice from 2 to 6 ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Oct","modification":"2025-04-18T12:53:59.124Z","creation":"2025-04-06T22:17:36.259Z"},"accession":"S-EPMC10834862","cross_references":{"pubmed":["37536656"],"doi":["10.1016/j.jtos.2023.07.012"]}}