biostudies-literatureLi WNEI NIH HHS17-41https://www.ebi.ac.uk/biostudies/studies/S-EPMC10834862biostudies-literatureUnknown30<h4>Purpose</h4>The conserved miR-183/96/182 cluster (miR-183C) regulates both corneal sensory innervation and corneal resident immune cells (CRICs). This study is to uncover its role in CRICs and in shaping the corneal cellular landscape at a single-cell (sc) level.<h4>Methods</h4>Corneas of naïve, young adult [2 and 6 months old (mo)], female miR-183C knockout (KO) mice and wild-type (WT) littermates were harvested and dissociated into single cells. Dead cells were removed using a Dead Cell Removal kit. CD45⁺ CRICs were enriched by Magnetic Activated Cell Sorting (MACS). scRNA libraries were constructed and sequenced followed by comprehensive bioinformatic analyses.<h4>Results</h4>The composition of major cell types of the cornea stays relatively stable in WT mice from 2 to 6 The ocular surfaceThe miR-183/96/182 cluster is a checkpoint for resident immune cells and shapes the cellular landscape of the cornea.PMC10834862R01 EY026059P30 EY004068R01 EY016058Li WGurdziel KPitchaikannu AHazlett LDXu SGupta NfalseThe miR-183/96/182 cluster is a checkpoint for resident immune cells and shapes the cellular landscape of the cornea.<h4>Purpose</h4>The conserved miR-183/96/182 cluster (miR-183C) regulates both corneal sensory innervation and corneal resident immune cells (CRICs). This study is to uncover its role in CRICs and in shaping the corneal cellular landscape at a single-cell (sc) level.<h4>Methods</h4>Corneas of naïve, young adult [2 and 6 months old (mo)], female miR-183C knockout (KO) mice and wild-type (WT) littermates were harvested and dissociated into single cells. Dead cells were removed using a Dead Cell Removal kit. CD45⁺ CRICs were enriched by Magnetic Activated Cell Sorting (MACS). scRNA libraries were constructed and sequenced followed by comprehensive bioinformatic analyses.<h4>Results</h4>The composition of major cell types of the cornea stays relatively stable in WT mice from 2 to 6 2023-01-01T00:00:00Z2023 Oct2025-04-18T12:53:59.124Z2025-04-06T22:17:36.259ZS-EPMC108348623753665610.1016/j.jtos.2023.07.012