biostudies-literatureUnknown14Khalil H<h4>Aim</h4>suppression of methylation inhibitors (epigenetic genes) in hepatocarcinogenesis induced by diethylnitrosamine using glycyrrhetinic acid.<h4>Method</h4>In the current work, we investigated the effect of sole GA combined with different agents such as doxorubicin (DOX) or probiotic bacteria (<i>Lactobacillus rhamanosus</i>) against hepatocarcinogenesis induced by diethylnitrosamine to improve efficiency. The genomic DNA was isolated from rats' liver tissues to evaluate either methylation-sensitive or methylation-dependent resection enzymes. The methylation activity of the targeting genes DLC-1, TET-1, NF-kB, and STAT-3 was examined using specific primers and cleaved DNA products. Furthermore, flow cytometry was used to determine the protein expression profiles of DLC-1 and TET-1 Frontiers in immunology1206990https://www.ebi.ac.uk/biostudies/studies/S-EPMC10844948biostudies-literatureAmelioration effect of 18β-Glycyrrhetinic acid on methylation inhibitors in hepatocarcinogenesis -induced by diethylnitrosamine.PMC10844948Ibrahim IAHassan AElmaksoud AIAMohamed DDKhalil HRijo PMahrous HAl-Salmi FANada AHfalseAmelioration effect of 18β-Glycyrrhetinic acid on methylation inhibitors in hepatocarcinogenesis -induced by diethylnitrosamine.<h4>Aim</h4>suppression of methylation inhibitors (epigenetic genes) in hepatocarcinogenesis induced by diethylnitrosamine using glycyrrhetinic acid.<h4>Method</h4>In the current work, we investigated the effect of sole GA combined with different agents such as doxorubicin (DOX) or probiotic bacteria (<i>Lactobacillus rhamanosus</i>) against hepatocarcinogenesis induced by diethylnitrosamine to improve efficiency. The genomic DNA was isolated from rats' liver tissues to evaluate either methylation-sensitive or methylation-dependent resection enzymes. The methylation activity of the targeting genes DLC-1, TET-1, NF-kB, and STAT-3 was examined using specific primers and cleaved DNA products. Furthermore, flow cytometry was used to determine the protein expression profiles of DLC-1 and TET-1 2023-01-01T00:00:00Z20232025-04-22T03:25:31.765Z2025-04-05T20:40:25.456ZS-EPMC108449483832201310.3389/fimmu.2023.1206990