biostudies-literatureOrmala-Tiznado A-MVetenskapsrådetVetenskapsrådet (VR)e0403622https://www.ebi.ac.uk/biostudies/studies/S-EPMC10845972biostudies-literatureUnknown12(2)Extensively drug-resistant (XDR) <i>Klebsiella pneumoniae</i> inflict a notable burden on healthcare worldwide. Of specific concern are strains producing carbapenem-hydrolyzing enzymes, as the therapeutic options for these strains are still very limited. Specific sequence types of <i>K. pneumoniae</i> have been noted for their epidemic occurrence globally, but the mechanisms behind the success of specific clones remain unclear. Herein, we have characterized 20 high-risk clones (HiRCs) and 10 non-HiRCs of XDR <i>K. pneumoniae</i>, exploring factors connected to the epidemiological success of some clones. Isolates were subjected to core genome multilocus sequence typing analysis to determine the clonal relationships of the isolates and subsequently characterized with regard to features knownMicrobiology spectrumMolecular characteristics, fitness, and virulence of high-risk and non-high-risk clones of carbapenemase-producing <i>Klebsiella pneumoniae</i>.PMC108459722016-02889Coorens MBrisse SSandegren LMaatallah MPatpatia SGiske CGAllander LKabir MHOrmala-Tiznado A-MfalseMolecular characteristics, fitness, and virulence of high-risk and non-high-risk clones of carbapenemase-producing <i>Klebsiella pneumoniae</i>.Extensively drug-resistant (XDR) <i>Klebsiella pneumoniae</i> inflict a notable burden on healthcare worldwide. Of specific concern are strains producing carbapenem-hydrolyzing enzymes, as the therapeutic options for these strains are still very limited. Specific sequence types of <i>K. pneumoniae</i> have been noted for their epidemic occurrence globally, but the mechanisms behind the success of specific clones remain unclear. Herein, we have characterized 20 high-risk clones (HiRCs) and 10 non-HiRCs of XDR <i>K. pneumoniae</i>, exploring factors connected to the epidemiological success of some clones. Isolates were subjected to core genome multilocus sequence typing analysis to determine the clonal relationships of the isolates and subsequently characterized with regard to features known2024-01-01T00:00:00Z2024 Feb2025-04-22T03:13:05.594Z2025-04-05T20:42:29.813ZS-EPMC108459723820595810.1128/spectrum.04036-22